Methodological Guidelines to Study Extracellular Vesicles

被引：905

作者：

Coumans, Frank A. W. ^{[1
,2
]}

Brisson, Alain R. ^{[4
]}

Buzas, Edit I. ^{[5
]}

Dignat-George, Francoise ^{[6
,7
]}

Drees, Esther E. E. ^{[8
]}

El-Andaloussi, Samir ^{[9
,10
]}

Emanueli, Costanza ^{[11
,12
]}

Gasecka, Aleksandra ^{[3
,13
]}

Hendrix, An ^{[14
,15
]}

Hill, Andrew F. ^{[16
]}

Lacroix, Romaric ^{[6
,7
]}

Lee, Yi ^{[9
]}

van Leeuwen, Ton G. ^{[1
,2
]}

Mackman, Nigel ^{[17
]}

Maeger, Imre ^{[10
,18
]}

Nolan, John P. ^{[19
]}

van der Pol, Edwin ^{[1
,2
]}

Pegtel, D. Michiel ^{[8
]}

Sahoo, Susmita ^{[20
]}

Siljander, Pia R. M. ^{[21
,22
,23
]}

Sturk, Guus ^{[2
,3
]}

de Wever, Olivier ^{[14
,15
]}

Nieuwland, Rienk ^{[2
,3
]}

机构：

[1] Univ Amsterdam, Acad Med Ctr, Biomed Engn & Phys, Amsterdam, Netherlands

[2] Univ Amsterdam, Acad Med Ctr, Vesicle Observat Ctr, Amsterdam, Netherlands

[3] Univ Amsterdam, Acad Med Ctr, Lab Expt Clin Chem, Amsterdam, Netherlands

[4] Univ Bordeaux, IPB, UMR 5248 CBMN CNRS, Extracellular Vesicles & Membrane Repair, Pessac, France

[5] Semmelweis Univ, Dept Genet, Cell & Immunobol, Budapest, Hungary

[6] Aix Marseille Univ, INSERM, VRCM, UFR Pharm,UMRS 1076, Marseille, France

[7] CHU La Concept, APHM, Haematol & Vasc Biol Dept, Marseille, France

[8] Vrije Univ Amsterdam Med Ctr, Dept Pathol, Exosomes Res Grp, Boelelaan 1117, Amsterdam, Netherlands

[9] Karolinska Inst, Dept Lab Med, Stockholm, Sweden

[10] Univ Oxford, Dept Physiol, Anat & Genet, Oxford, England

[11] Univ Bristol, Bristol Heart Inst, Bristol, Avon, England

[12] Natl Heart Lung Inst, Imperial Coll London, London, England

[13] Med Univ Warsaw, Chair & Dept Cardiol 1, Warsaw, Poland

[14] Univ Ghent, Dept Radiat Oncol & Expt Canc Res, Lab Expt Canc, Ghent, Belgium

[15] Canc Res Inst, Ghent, Belgium

[16] La Trobe Univ, La Trobe Inst Mol Sci, Dept Biochem & Genet, Bundoora, Vic, Australia

[17] Univ North Carolina Chapel Hill, Dept Med, Chapel Hill, NC USA

[18] Univ Tartu, Inst Technol, Tartu, Estonia

[19] Scintillon Inst, San Diego, CA USA

[20] Icahn Sch Med, Cardiovasc Res Ctr, New York, NY USA

[21] Univ Helsinki, EV Core Facil, Helsinki, Finland

[22] Univ Helsinki, Div Biochem & Biotechnol, EV Grp, Helsinki, Finland

[23] Univ Helsinki, Dept Biosci, Helsinki, Finland

来源：

CIRCULATION RESEARCH | 2017年 / 120卷 / 10期

关键词：

cardiovascular diseases; extracellular vesicles; exosomes; methods; reference standards; TISSUE FACTOR ACTIVITY; NANOPARTICLE TRACKING ANALYSIS; CELL-DERIVED MICROPARTICLES; ATOMIC-FORCE MICROSCOPY; FLOW-CYTOMETRY; INTERNATIONAL SOCIETY; TRANSFERRIN RECEPTOR; ENDOTHELIAL-CELLS; FETAL BOVINE; CIRCULATING MICROPARTICLES;

D O I：

10.1161/CIRCRESAHA.117.309417

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

引用

页码：1632 / 1648

页数：17

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