Single surface stabilizer

被引：65

作者：

Pace, CN ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Med Biochem & Genet, College Stn, TX 77845 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2000年 / 7卷 / 05期

关键词：

D O I：

10.1038/75100

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enhanced stability of a cold shock protein from a thermophilic organism, compared to its counterpart from a mesophile, results mainly from a single change in amino acid sequence that improves electrostatic interactions among the charged groups on the protein surface.

引用

页码：345 / 346

页数：2

共 17 条

[1] CUMULATIVE SITE-DIRECTED CHARGE-CHANGE REPLACEMENTS IN BACTERIOPHAGE-T4 LYSOZYME SUGGEST THAT LONG-RANGE ELECTROSTATIC INTERACTIONS CONTRIBUTE LITTLE TO PROTEIN STABILITY [J].

DAOPIN, S ;

SODERLIND, E ;

BAASE, WA ;

WOZNIAK, JA ;

SAUER, U ;

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (03) :873-887

[2] Molecular dynamics simulations of the hyperthermophilic protein Sac7d from Sulfolobus acidocaldarius:: Contribution of salt bridges to thermostability [J].

de Bakker, PIW ;

Hünenberger, PH ;

McCammon, JA .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 285 (04) :1811-1830

[3] The stability of salt bridges at high temperatures: Implications for hyperthermophilic proteins [J].

Elcock, AH .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 284 (02) :489-502

[4] Increasing protein stability by altering long-range coulombic interactions [J].

Grimsley, GR ;

Shaw, KL ;

Fee, LR ;

Alston, RW ;

Huyghues-Despointes, BMP ;

Thurlkill, RL ;

Scholtz, JM ;

Pace, CN .

PROTEIN SCIENCE, 1999, 8 (09) :1843-1849

[5] Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species [J].

Haney, PJ ;

Badger, JH ;

Buldak, GL ;

Reich, CI ;

Woese, CR ;

Olsen, GJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) :3578-3583

[6] The stability of proteins in extreme environments [J].

Jaenicke, R ;

Böhm, G .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1998, 8 (06) :738-748

[7] Proteins from thermophilic and mesophilic organisms essentially do not differ in packing [J].

Karshikoff, A ;

Ladenstein, R .

PROTEIN ENGINEERING, 1998, 11 (10) :867-872

[8] Engineering a thermostable protein via optimization of charge-charge interactions on the protein surface [J].

Loladze, VV ;

Ibarra-Molero, B ;

Sanchez-Ruiz, JM ;

Makhatadze, GI .

BIOCHEMISTRY, 1999, 38 (50) :16419-16423

[9] Contributions of the ionizable amino acids to the stability of staphylococcal nuclease [J].

Meeker, AK ;

GarciaMoreno, B ;

Shortle, D .

BIOCHEMISTRY, 1996, 35 (20) :6443-6449

[10]

Perl D, 2000, NAT STRUCT BIOL, V7, P380

← 1 2 →