Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1

被引：23

作者：

Fernández, MJ

Adrio, JL

Piret, JM

Wolfe, S

Ro, S

Demain, AL ^{[1
]}

机构：

[1] MIT, Dept Biol, Cambridge, MA 02139 USA

[2] Northeastern Univ, Dept Biol, Boston, MA 02115 USA

[3] Simon Fraser Univ, Dept Chem, Burnaby, BC V5A 1S6, Canada

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1999年 / 52卷 / 04期

关键词：

D O I：

10.1007/s002530051549

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Growth of Streptomyces clavuligerus NP1 in the presence of methanol or ethanol resulted in a marked increase in production of cephalosporin(s) from penicillin G by resting cells. The mycelium produced in alcohol-supplemented medium was fragmented and dispersed as compared with growth in control medium. HPLC analysis showed that at least two products were present in the biotransformation supernatant fluid after 1 h incubation. One of them has been identified as deacetoxycephalosporin G (DAOG).

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页码：484 / 488

页数：5

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