A novel inhibitor of bacterial endotoxin derived from cinnamon bark

被引:37
作者
Azumi, S
Tanimura, A
Tanamoto, KI
机构
[1] NATL INST HLTH SCI,DIV MICROBIOL,TOKYO 158,JAPAN
[2] SHOWA WOMENS UNIV,TOKYO 154,JAPAN
关键词
D O I
10.1006/bbrc.1997.6668
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A substance that inhibits the activity of bacterial endotoxin (LPS) was found in cinnamon bark. The inhibitor, extracted from dry cinnamon bark with 67% ethanol/water, was purified by using Limulus gelation activity as an indicator of endotoxin activity. The inhibitor suppressed the activity of the LPS when it was mixed with the inhibitor prior to the assay. The reduction of the LPS activity depended on the concentration of both the inhibitor and LPS when mixed, and also on the incubation time. The inhibitor suppressed the activity of all LPS and lipid A preparations tested regardless of the origin of the bacteria. The inhibitor alone did not affect the Limulus system. These results indicate that the inhibition was caused by direct interaction of the inhibitor with the LPS molecule. Furthermore the inhibitor abrogated the pyrogenicity of the LPS. Although it is uncertain whether the inhibitor actually plays a role in the defense mechanism in cinnamon bark, this is the first report that an inhibitor of bacterial endotoxin exists in a plant. (C) 1997 Academic Press.
引用
收藏
页码:506 / 510
页数:5
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