Purification and characterization of alginate lyase from marine Vibrio sp. YWA

被引：17

作者：

Wang, Yuan-Hong ^{[1
]}

Yu, Guang-Li ^{[1
]}

Wang, Xin-Min ^{[1
]}

Lv, Zhi-Hua ^{[1
]}

Zhao, Xia ^{[1
]}

Wu, Zhi-Hong ^{[1
]}

Ji, Wei-Shang ^{[1
]}

机构：

[1] Ocean Univ China, Marine Drug & Food Inst, Key Lab Marine Drugs Shandong Province, Key Lab Marine Drugs,Minist Educ, Qingdao 266003, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2006年 / 38卷 / 09期

关键词：

marine Vibrio; alginate; alginate lyase; purification; characterization;

D O I：

10.1111/j.1745-7270.2006.00210.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular alginate lyase secreted by marine Vibrio sp. YWA, isolated from decayed Laminaria japonica, was purified by a combination of ammonium sulfate precipitation and diethylaminoethyl-Sephacel column chromatography. The results show that the molecular mass of alginate lyase was approximately 62.5 kDa, with an optimal pH and temperature at pH 7.0 and 25 degrees C, respectively. Km was approximately 72.73 g/L. The activity of the enzyme was enhanced by EDTA and Zn2+, but inhibited by Ba2+. The substrates specificity analysis shows that it was specific for hydrolyzing poly-beta-D-1,4-mannuronate in alginate.

引用

页码：633 / 638

页数：6

共 21 条

[1] EFFECT OF DEPOLYMERIZED ALGINATES ON THE GROWTH OF BIFIDOBACTERIA [J].