Labeling proteins at high specific activity using N-succinimidyl 4-[F-18](fluoromethyl) benzoate

被引：38

作者：

Lang, L

Eckelman, WC

机构：

[1] Positron Emission Tomography Dept., Warren Grant Magnuson Clin. Center, National Institutes of Health, Bethesda, MD 20892

[2] NIH, Building 10, Bethesda, MD 20892

来源：

APPLIED RADIATION AND ISOTOPES | 1997年 / 48卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0969-8043(96)00151-0

中图分类号：

O61 [无机化学];

学科分类号：

070301 [无机化学]; 081704 [应用化学];

摘要：

High effective specific activity N-succinimidyl 4-[F-18](fluoromethyl)benzoate was prepared using a reversed phase HPLC procedure. Reversed phase HPLC removed several additional impurities not removed by normal phase HPLC, thereby increasing the effective specific activity. Small amount (<100 mu g) of sensitive proteins such as erythropoietin can be labeled with this reagent in high yield without aggregation. Copyright (C) 1997 Published by Elsevier Science Ltd

引用

页码：169 / 173

页数：5

共 16 条

[1]

Aloj L, 1996, J NUCL MED, V37, P1408

[2]

ERYTHROPOIETIN RECEPTOR CHARACTERISTICS ON PRIMARY HUMAN ERYTHROID-CELLS [J].

BROUDY, VC ;

LIN, N ;

BRICE, M ;

NAKAMOTO, B ;

PAPAYANNOPOULOU, T .

BLOOD, 1991, 77 (12) :2583-2590

[3]

CHITAMBAR CR, 1987, CANCER RES, V47, P3929

[4]

CHRETIEN S, 1994, BLOOD, V83, P1813

[5]

THE APPLICATION OF RECEPTOR THEORY TO RECEPTOR-BINDING AND ENZYME-BINDING ONCOLOGIC RADIOPHARMACEUTICALS [J].

ECKELMAN, WC .

NUCLEAR MEDICINE AND BIOLOGY, 1994, 21 (05) :759-769

[6]

EQUILIBRIUM-CONSTANTS FOR THE BINDING OF INDIUM(III) TO HUMAN SERUM TRANSFERRIN [J].

HARRIS, WR ;

CHEN, Y ;

WEIN, K .

INORGANIC CHEMISTRY, 1994, 33 (22) :4991-4998

[7]

KREITMAN RJ, 1994, BLOOD, V83, P426

[8]

ONE-STEP SYNTHESIS OF F-18 LABELED [F-18] N-SUCCINIMIDYL 4-(FLUOROMETHYL)BENZOATE FOR PROTEIN LABELING [J].

LANG, LX ;

ECKELMAN, WC .

APPLIED RADIATION AND ISOTOPES, 1994, 45 (12) :1155-1163

[9]

LARSON SM, 1979, J NUCL MED, V20, P837

[10]

LARSON SM, 1980, JNCI-J NATL CANCER I, V64, P41

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