Automated mounting, centering and screening of crystals for high-throughput protein crystallography

被引：53

作者：

Karain, WI

Bourenkov, GP

Blume, H

Bartunik, HD

机构：

[1] DESY, MPG ASMB, Max Planck Res Unit Struct Mol Biol, D-2000 Hamburg, Germany

[2] Birzeit Univ, Dept Phys, Birzeit, Israel

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷

关键词：

structural genomics; crystal mounting robot; synchrotron radiation;

D O I：

10.1107/S0907444902012751

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fully automated system for screening protein crystals for X-ray diffraction analysis has been designed and is being installed on the beamline BW6 at DORIS in Hamburg, Germany. The system includes robotic mounting of flash-frozen crystals from a storage dewar, centering and alignment of the sample both by optical and Xray (scattering and fluorescence) techniques, assessment of the diffraction quality of the sample, and SAD/MAD or nonconventional diffraction data acquisition with high-throughput data rates. The system covers all experimental steps required for protein x-ray structure analysis and provides a powerful means for structural genomics projects.

引用

页码：1519 / 1522

页数：4

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