Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription polymerase chain reaction targeting cytokeratin-20 mRNA

被引：53

作者：

Funaki, NO ^{[1
]}

Tanaka, JJ ^{[1
]}

Itami, A ^{[1
]}

Kasamatsu, T ^{[1
]}

Ohshio, GJ ^{[1
]}

Onodera, H ^{[1
]}

Monden, K ^{[1
]}

Okino, T ^{[1
]}

Imamura, M ^{[1
]}

机构：

[1] SHIGA MED CTR ADULT DIS,DEPT SURG,SHIGA,JAPAN

来源：

LIFE SCIENCES | 1997年 / 60卷 / 09期

关键词：

cytokeratin-20; mRNA; RT-PCR; colorectal carcinoma; hematogenous dissemination;

D O I：

10.1016/S0024-3205(96)00700-X

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

For the detection of circulating colorectal carcinoma cells, we investigated the presence of cytokeratin 20 (CK 20) mRNA in the peripheral blood of colorectal carcinoma patients. Application of our published technique resulted in analysis by reverse transcription followed by three-step nested polymerase chain reaction. This analysis could detect a single Cole 205 colon cancer cell mixed with 1 mi of blood. Our system also successfully detected the presence of CK 20 mRNA in actual patients' peripheral blood samples. Our highly sensitive and specific system for the detection of CK-20 mRNA from patients' peripheral blood thus seems to be useful for screening for circulating colorectal carcinoma cells.

引用

收藏

页码：643 / 652

页数：10

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