Simultaneous Separation and Determination of Different Polar Flavonoids in Multiflora Fruit by Reverse-Phase High-Performance Liquid Chromatography

被引：5

作者：

Dilinuer, Malike ^{[1
,2
]}

Sun, Binjie ^{[1
]}

Baeyens, Willy R. G. ^{[3
]}

Ouyang, Jin ^{[1
]}

机构：

[1] Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China

[2] Xinjiang Normal Univ, Coll Life & Environm Sci, Urumqi, Peoples R China

[3] Univ Ghent, Fac Pharmaceut Sci, Dept Pharmaceut Anal, B-9000 Ghent, Belgium

来源：

ANALYTICAL LETTERS | 2009年 / 42卷 / 08期

基金：

北京市自然科学基金;

关键词：

Flavonoids; fruit analysis; gradient elution; mobile phase composition; reverse-phase HPLC; PRUNUS-CERASUS L; TART CHERRIES; CAPILLARY-ELECTROPHORESIS; MASS SPECTROMETRY; ANTHOCYANINS; EXTRACTION; QUERCETIN; PLASMA; RUTIN;

D O I：

10.1080/00032710902890397

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A method for the determination of different polar flavonoids from multiflora fruit by reverse-phase high-performance liquid chromatography (HPLC) is presented in this article. Chromatographic separation of these flavonoids was performed over a Diamonsil C18 column (200mm4.6mm5m) using gradient elution with an isopropanol-methanol-water mixture at 25C, at a flow rate ranging from 0.5 to 1.5mL/min and detection at 360nm. The flavonoids could be separated within 6min; the recovery was between 96% and 104%, and the relative standard deviation was 1.6-2.6%. This method allows rapid detection of flavonoids.

引用

页码：1136 / 1147

页数：12

共 19 条

[1]

Flavonoid and chlorogenic acid concentrations in skin of 'Jonagold' and 'Elstar' apples during and after regular and ultra low oxygen storage [J].

Awad, MA ;

de Jager, A .

POSTHARVEST BIOLOGY AND TECHNOLOGY, 2000, 20 (01) :15-24

[2]

Flavonoid and chlorogenic acid levels in apple fruit: characterisation of variation [J].

Awad, MA ;

de Jager, A ;

van Westing, LM .

SCIENTIA HORTICULTURAE, 2000, 83 (3-4) :249-263

[3]

SIMULTANEOUS ISOLATION OF RUTIN AND ESCULIN FROM PLANT-MATERIAL AND DRUGS USING SOLID-PHASE EXTRACTION [J].

BUSZEWSKI, B ;

KAWKA, S ;

SUPRYNOWICZ, Z ;

WOLSKI, T .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1993, 11 (03) :211-215

[4]

Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships [J].

Cao, GH ;

Sofic, E ;

Prior, RL .

FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (05) :749-760

[5]

ISOLATION AND STABILIZATION OF ANTHOCYANINS FROM TART CHERRIES (PRUNUS-CERASUS L) [J].

CHANDRA, A ;

NAIR, MG ;

IEZZONI, AF .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (07) :1062-1065

[6]

EVALUATION AND CHARACTERIZATION OF THE ANTHOCYANIN PIGMENTS IN TART CHERRIES (PRUNUS-CERASUS L) [J].

CHANDRA, A ;

NAIR, MG ;

IEZZONI, A .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (06) :967-969

[7]

CHEN FZ, 2001, J INTEGR PLANT BIOL, V43, P101

[8]

Analysis of anthocyanins in foods by liquid chromatography, liquid chromatography-mass spectrometry and capillary electrophoresis [J].

da Costa, CT ;

Horton, D ;

Margolis, SA .

JOURNAL OF CHROMATOGRAPHY A, 2000, 881 (1-2) :403-410

[9]

Gas chromatography-mass spectrometry method for the simultaneous determination of wood extractive compounds in quaking aspen [J].

Fernandez, MP ;

Watson, PA ;

Breuil, C .

JOURNAL OF CHROMATOGRAPHY A, 2001, 922 (1-2) :225-233

[10]

Determination of rutin in human plasma by high-performance liquid chromatography utilizing solid-phase extraction and ultraviolet detection [J].

Ishii, K ;

Furuta, T ;

Kasuya, Y .

JOURNAL OF CHROMATOGRAPHY B, 2001, 759 (01) :161-168

← 1 2 →