High-performance liquid chromatography with photodiode array detection (HPLC-DAD)/HPLC-mass spectrometry (MS) profiling of anthocyanins from Andean mashua tubers (Tropaeolum tuberosum Ruiz and Pavon) and their contribution to the overall antioxidant activity

被引:30
作者
Chirinos, Rosana
Campos, David
Betalleluz, Indira
Giusti, M. Monica
Schwartz, Steven J.
Tian, Qingguo
Pedreschi, Romina
Larondelle, Yvan
机构
[1] Inst Sci Vie, Unite Biochim Nutr, B-1348 Louvain, Belgium
[2] Catholic Univ Louvain, B-1348 Louvain, Belgium
[3] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA
[4] Univ Nacl Agraria La Molina, Inst Biotecnol, Lima, Peru
关键词
mashua; anthocyanins; Tropaeolum tuberosum; antioxidant activity; HPLC-DAD; HPLC-ESI/MS-MS; total phenolics;
D O I
10.1021/jf0614140
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Mashua (Tropaeolum tuberosum Ruiz and Pavon), an Andean tuber with high antioxidant activity, has sparked interest because of its traditional medicinal use. In this study, we evaluated the anthocyanin composition for three purple mashua genotypes and their contribution to the overall antioxidant activity of the tuber. Mashua anthocyanins, total phenolics, and 2,2 '-azinobis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant activity ranged from 45.5 to 131.9 mg of cyanidin 3-glucoside equivalents/100 g fresh weight (FW), 174.9 to 275.5 mg of gallic acid equivalents/ 100 g of FW, and 16.2 to 45.7 mu mol of Trolox equivalents/ g of FW, respectively. The high-performance liquid chromatography with photodiode array detection (HPLC-DAD) and HPLC-electrospray ionization tandem mass spectrometry (ESI/MS-MS) profiles revealed the presence of 11 different anthocyanins. The two major pigments (56.4-73.0% total area range at 520 nm) were identified as delphinidin 3-glucoside-5-acetylrhamnoside and delphinidin 3-sophoroside-5-acetylrhamnoside. Other pigments were delphinidin 3-glucoside-5-rhamnoside, delphinidin 3-sophoroside-5-rhamnoside, delphinidin 3-glucoside, cyanidin 3-sophoroside, and cyanidin 3-sophoroside-5-rhamnoside. Cyanidin 3-glucoside and cyanidin 3-rutinoside were only found in two genotypes, while pelargonidin 3-sophoroside and pelargonidin 3-sophoroside-5-rhamnoside were only found in the third one. Anthocyanins from mashua were the major contributors to the total ABTS values for only one of the three genotypes, suggesting that other phenolics present are playing a major role in the antioxidant power of mashua tubers. Results from this study provide important information for the Nutraceutical and Functional Food Market for the use of mashua anthocyanins not only as a source of natural colorants but also as a source of phytonutrients.
引用
收藏
页码:7089 / 7097
页数:9
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