Nup211, the fission yeast homolog of Mlp1/Tpr, is involved in mRNA export

被引：8

作者：

Bae, Jin-Ah ^{[2
]}

Moon, DongGeRaMi ^{[1
,3
]}

Yoon, Jin Ho ^{[1
,3
]}

机构：

[1] Sungshin Womens Univ, Sch Biol Sci & Chem, Seoul 136742, South Korea

[2] Sungshin Womens Univ, Dept Educ, Seoul 136742, South Korea

[3] Sungshin Womens Univ, Basic Sci Res Inst, Seoul 136742, South Korea

来源：

JOURNAL OF MICROBIOLOGY | 2009年 / 47卷 / 03期

关键词：

mRNA export; Schizosaccharomyces pombe; nup211; NUCLEAR-PORE COMPLEX; SCHIZOSACCHAROMYCES-POMBE; SACCHAROMYCES-CEREVISIAE; GENETICALLY INTERACTS; POLY(A)(+) RNA; EXPRESSION; MEX67P; TPR; PROTEINS; VECTORS;

D O I：

10.1007/s12275-009-0125-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Synthetic lethal mutants have been previously isolated in fission yeast Schizosaccharomyces pombe, which genetically interact with spmex67, in order to identify the genes involved in mRNA export. The nup211 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex2, under synthetic lethal condition. We showed that Nup211, fission yeast homolog of Mlpl/Mlp2/Tpr, is essential for vegetative growth and Nup211-GFP proteins expressed at endogenous level are localized mainly in nuclear periphery. The accumulation of poly(A)(+) RNA in the nucleus is exhibited when expression of nup211 is repressed or over-expressed. These results suggest that the Nup211 protein plays a pivotal role of mRNA export in fission yeast.

引用

页码：337 / 343

页数：7

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