A second-generation integrated map of the silkworm reveals synteny and conserved gene order between lepidopteran insects

被引：58

作者：

Yasukochi, Yuji

Ashakumary, Laksmikutty A.

Baba, Kotaro

Yoshido, Atsuo

Sahara, Ken

机构：

[1] Natl Inst Agrobiol Sci, Insect Genome Lab, Tsukuba, Ibaraki 3058634, Japan

[2] Hokkaido Univ, Grad Sch Agr, Div Appl Biosci, Sapporo, Hokkaido 0608589, Japan

来源：

GENETICS | 2006年 / 173卷 / 03期

关键词：

D O I：

10.1534/genetics.106.055541

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A second-generation linkage map was constructed for the silkworm, Bombyx mori, focusing on mapping Bombyx sequences appearing in public nucleotide databases and bacterial artificial chromosome (BAC) contigs. A total of 874 BAC contigs containing 5067 clones (22% of the library) were constructed by PCR-based screening with sequence-tagged sites (STSs) derived from whole-genome shotgun (WGS) sequences. A total of 523 BAC contigs, including 342 independent genes registered in public databases and 85 expressed sequence tags (ESTs), were placed onto the linkage map. We found significant synteny and conserved gene order between B. mori and a nymphalid butterfly, Heliconius melpomene, in four linkage groups (LGs), strongly suggesting that using B. mori as a reference for comparative genomics in Lepidotera is highly feasible.

引用

页码：1319 / 1328

页数：10

共 25 条

[1]

Banno YF., 2005, GUIDE SILKWORM MUTAN

[2] Mining single nucleotide polymorphisms from EST data of silkworm, Bombyx mori, inbred strain Dazao [J].

Cheng, TC ;

Xia, QY ;

Qian, JF ;

Liu, C ;

Lin, Y ;

Zha, XF ;

Xiang, ZH .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2004, 34 (06) :523-530

[3] The genetics and genomics of the silkworm, Bombyx mori [J].

Goldsmith, MR ;

Shimada, T ;

Abe, H .

ANNUAL REVIEW OF ENTOMOLOGY, 2005, 50 :71-100

[4]

HUNT GJ, 1995, GENETICS, V139, P1371

[5] A genetic linkage map of the mimetic butterfly Heliconius melpomene [J].

Jiggins, CD ;

Mavarez, J ;

Beltrán, M ;

McMillan, WO ;

Johnston, JS ;

Bermingham, E .

GENETICS, 2005, 171 (02) :557-570

[6]

Lander ES., 1987, GENOMICS, V16, P224

[7] Genetic linkage maps of the red hour beetle, Tribolium castaneum, based on bacterial artificial chromosomes and expressed sequence tags [J].

Lorenzen, MD ;

Doyungan, Z ;

Savard, J ;

Snow, K ;

Crumly, LR ;

Shippy, TD ;

Stuart, JJ ;

Brown, SJ ;

Beeman, RW .

GENETICS, 2005, 170 (02) :741-747

[8] Simple sequence repeat-based consensus linkage map of Bombyx mori [J].

Miao, XX ;

Xu, SJ ;

Li, MH ;

Li, MW ;

Huang, JH ;

Dai, FY ;

Marino, SW ;

Mills, DR ;

Zeng, PY ;

Mita, K ;

Jia, SH ;

Zhang, Y ;

Liu, WB ;

Xiang, H ;

Guo, QH ;

Xu, AY ;

Kong, XY ;

Lin, HX ;

Shi, YZ ;

Lu, G ;

Zhang, XL ;

Huang, W ;

Yasukochi, Y ;

Sugasaki, T ;

Shimada, T ;

Nagaraju, J ;

Xiang, ZH ;

Wang, SY ;

Goldsmith, MR ;

Lu, C ;

Zhao, GP ;

Huang, YP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (45) :16303-16308

[9] The genome sequence of silkworm, Bombyx mori [J].

Mita, K ;

Kasahara, M ;

Sasaki, S ;

Nagayasu, Y ;

Yamada, T ;

Kanamori, H ;

Namiki, N ;

Kitagawa, M ;

Yamashita, H ;

Yasukochi, Y ;

Kadono-Okuda, K ;

Yamamoto, K ;

Ajimura, M ;

Ravikumar, G ;

Shimomura, M ;

Nagamura, Y ;

Shin-I, T ;

Abe, H ;

Shimada, T ;

Morishita, S ;

Sasaki, T .

DNA RESEARCH, 2004, 11 (01) :27-35

[10] The construction of an EST database for Bombyx mori and its application [J].

Mita, K ;

Morimyo, M ;

Okano, K ;

Koike, Y ;

Nohata, J ;

Kawasaki, H ;

Kadono-Okuda, K ;

Yamamoto, K ;

Suzuki, MG ;

Shimada, T ;

Goldsmith, MR ;

Maeda, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (24) :14121-14126

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