Performance of Microcalorimetry for Early Detection of Methicillin Resistance in Clinical Isolates of Staphylococcus aureus

被引：71

作者：

Baldoni, Daniela ^{[3
]}

Hermann, Heinz ^{[3
]}

Frei, Reno ^{[4
]}

Trampuz, Andrej ^{[1
,2
,3
]}

Steinhuber, Andrea ^{[3
]}

机构：

[1] Univ Basel Hosp, Div Infect Dis, CH-4031 Basel, Switzerland

[2] Univ Basel Hosp, Hosp Epidemiol, CH-4031 Basel, Switzerland

[3] Univ Basel Hosp, Dept Biomed, Infect Dis Res Lab, CH-4031 Basel, Switzerland

[4] Univ Basel Hosp, Lab Med, Microbiol Lab, CH-4031 Basel, Switzerland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 03期

基金：

瑞士国家科学基金会;

关键词：

EXPRESSION; CEFOXITIN; MRSA;

D O I：

10.1128/JCM.02374-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

We describe a calorimetric assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA) within 5 h. Microbial heat was calculated in culture with and without cefoxitin. Among 30 genetically distinct clinical isolates, 19/20 MRSA (95%) and 10/10 methicillin-susceptible Staphylococcus aureus ( 100%) were correctly identified. Microcalorimetry may be useful for rapid MRSA screening.

引用

页码：774 / 776

页数：3

共 14 条

[1]

Amsterdam D., 2005, Antibiotic s in Laboratory Medicine, V5th Edtn, P61

[2]

Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA) [J].

Brown, DFJ ;

Edwards, DI ;

Hawkey, PM ;

Morrison, D ;

Ridgway, GL ;

Towner, KJ ;

Wren, MWD .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 56 (06) :1000-1018

[3]

Use of cefoxitin-based selective broth for improved detection of methicillin-resistant Staphylococcus aureus [J].

Fang, H ;

Hedin, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (02) :592-594

[4]

Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA):: a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test [J].

Felten, A ;

Grandry, B ;

Lagrange, PH ;

Casin, I .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (08) :2766-2771

[5]

EVIDENCE THAT THE NATIONAL-COMMITTEE-FOR-CLINICAL-LABORATORY-STANDARDS DISK TEST IS LESS SENSITIVE THAN THE SCREEN PLATE FOR DETECTION OF LOW-EXPRESSION-CLASS METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS [J].

MACKENZIE, AMR ;

RICHARDSON, H ;

LANNIGAN, R ;

WOOD, D .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (07) :1909-1911

[6]

KINETICS OF ACTIONS OF TETRACYCLINES ON ESCHERICHIA-COLI AS STUDIED BY MICROCALORIMETRY [J].

MARDH, PA ;

RIPA, T ;

ANDERSSON, KE ;

WADSO, I .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1976, 10 (04) :604-609

[7]

Role of mecA transcriptional regulation in the phenotypic expression of methicillin resistance in Staphylococcus aureus [J].

Niemeyer, DM ;

Pucci, MJ ;

Thanassi, JA ;

Sharma, VK ;

Archer, GL .

JOURNAL OF BACTERIOLOGY, 1996, 178 (18) :5464-5471

[8]

Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus:: a prospective study comparing 32 methods [J].

Safdar, N ;

Narans, L ;

Gordon, B ;

Maki, DG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) :3163-3166

[9]

Spink C, 1976, Methods Biochem Anal, V23, P1, DOI 10.1002/9780470110430.ch1

[10]

Molecular typing of methicillin-resistant Staphylococcus aureus:: Can PCR replace pulsed-field gel electrophoresis? [J].

Strandén, A ;

Frei, R ;

Widmer, AF .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) :3181-3186

← 1 2 →