Tracking of Cellular Uptake of Hydrophilic CdSe/ZnS Quantum Dots/Hydroxyapatite Composites Nanoparticles in MC3T3-E1 Osteoblast Cells

被引：25

作者：

Hsieh, Ming-Fa ^{[1
,2
]}

Li, Jimmy Kuan-Jung ^{[3
]}

Lin, Cheng-An J. ^{[1
,2
]}

Huang, Shin-Huei ^{[1
,2
]}

Sperling, Ralph A. ^{[4
]}

Parak, Wolfgang J. ^{[4
]}

Chang, Walter H. ^{[1
,2
]}

机构：

[1] Chung Yuan Christian Univ, Dept Biomed Engn, R&D Ctr Membrane Technol, Chungli 32023, Taiwan

[2] Chung Yuan Christian Univ, Ctr Nano Bioengn, Chungli 32023, Taiwan

[3] Natl Cheng Kung Univ, Ctr Micronano Sci & Technol, Tainan 701, Taiwan

[4] Univ Marburg, Fachbereich Phys, D-35037 Marburg, Germany

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2009年 / 9卷 / 04期

关键词：

CdSe/ZnS Quantum Dots; Hydroxyapatite; Cellular Uptake; Osteoblast Cells; HYDROXYAPATITE PARTICLE-SIZE; IN-VITRO; NANO-HYDROXYAPATITE; CALCIUM-PHOSPHATE; TITANIUM; BONE; NANOCRYSTALS; FIBROBLASTS; CRYSTALS; COATINGS;

D O I：

10.1166/jnn.2009.463

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

We report the fluorescent labeling of osteoblast cells using the biocompatible hydroxyapatite (HA) grown with nucleating seed of hydrophilic CdSe/ZnS quantum dots (QDs) allowing the real-time observation of cell under confocal microscope. We found that the MC3T3-E1 osteoblast cells can engulf HA with surface-tailored QDs showing fluorescent spots in the cytoplasm, while HA and QDs nanoparticles were not engulfed. It is interesting to see that the fluorescence was only displayed in the cytoplasm of MC3T3-E1 osteoblast cells. It can be envisioned that the nano-sized hydroxyapatite bearing fluorescent QD can only be internalized in the cytoplasm. Therefore, it is worth utilizing these composite particles to observe cellular physiology with minimal toxicity to the osteoblast cells.

引用

页码：2758 / 2762

页数：5

共 30 条

[1]

The effects of combined gentamicin-hydroxyapatite coating for cementless joint prostheses on the reduction of infection rates in a rabbit infection prophylaxis model [J].