Cloning and characterization of the exbB-exbD-tonB locus of Pasteurella haemolytica A1

被引：8

作者：

Graham, MR ^{[1
]}

Lo, RYC ^{[1
]}

机构：

[1] UNIV GUELPH, DEPT MICROBIOL, GUELPH, ON N1G 2W1, CANADA

来源：

GENE | 1997年 / 186卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

tonB; gram-negative bacteria; gene cloning; complementation; periplasmic protein;

D O I：

10.1016/S0378-1119(96)00703-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A recombinant plasmid (pMG1) carrying Pasteurella haemolytica A1 DNA which complements a tonB mutation of Escherichia coli has been isolated. E. coli tonB metE which carries pMG1 exhibits growth kinetics in the presence of vitamin B-12 similar to that of the wild-type host. In addition, the complemented E. coli is susceptible to killing by bacteriophage phi 80 and colicin B. Analysis of the nucleotide sequence in the complementing DNA showed that it codes for three genes in the order of exbB-exbD-tonB. This genetic organization has been reported in Haemophilus influenzae, H. ducreyi, Pseudomonas putida and Vibrio cholerae, and may represent a separate lineage of evolution from that of the Enterobacteriaceae in which tonB is unlinked with the accessory genes exbB and exbD. A comparison of the DNA flanking the exbB-exbD-tonB locus in P. haemolytica A1 and H. influenzae showed that the flanking regions are completely different between the two organisms.

引用

页码：201 / 205

页数：5

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