Requirement of monooxygenase-mediated steps for sterigmatocystin biosynthesis by Aspergillus nidulans

被引：34

作者：

Keller, NP ^{[1
]}

Watanabe, CMH

Kelkar, HS

Adams, TH

Townsend, CA

机构：

[1] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA

[2] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

[3] Johns Hopkins Univ, Dept Chem, Baltimore, MD 21218 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 01期

关键词：

D O I：

10.1128/AEM.66.1.359-362.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sterigmatocystin (ST) and aflatoxin B-1 (AFB(1)) are two polyketide-derived Aspergillus mycotoxins synthesized by functionally identical sets of enzymes, ST, the compound produced by Aspergillus nidulans, is a late intermediate in the AFB(1) pathway of A. parasiticus and A. flavus. Previous biochemical studies predicted that five oxygenase steps are required for the formation of ST. A 60-kb ST gene cluster in A. nidulans contains five genes, stcB, stcF, stcL, stcS, and stcW; encoding putative monooxygenase activities. Prior research showed that stcL and stcS mutants accumulated versicolorins B and Al respectively. We now show that strains disrupted at stcF, encoding a P-450 monooxygenase similar to A. parasiticus avnA, accumulate averantin, Disruption of either StcB (a putative P-450 monooxygenase) or StcW (a putative flavin-requiring monooxygenase) led to the accumulation of averufin as determined by radiolabeled feeding and extraction studies.

引用

页码：359 / 362

页数：4

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