Cytotoxicity and DNA damage properties of tert-butylhydroquinone (TBHQ) food additive

被引：130

作者：

Eskandani, Morteza ^{[1
]}

Hamishehkar, Named ^{[2
]}

Dolatabadi, Jafar Ezzati Nazhad ^{[1
]}

机构：

[1] Tabriz Univ Med Sci, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran

[2] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

来源：

FOOD CHEMISTRY | 2014年 / 153卷

关键词：

Tert-butylhydroquinone (TBHQ); Apoptosis; A549; Comet assay; Flow cytometry; DAPi staining assay; 2-TERT-BUTYLHYDROQUINONE TBHQ; BUTYLATED HYDROXYANISOLE; METABOLITE; CELLS; OIL;

D O I：

10.1016/j.foodchem.2013.12.087

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Tert-butylhydroquinone (TBHQ) was tested for potential cytotoxicity and genotoxicity upon A549 lung cancer cells and Human Umbilical Vein Endothelial Cells (HUVEC). Cytotoxicity was evaluated by MIT assay and flow cytometry analysis, while genotoxicity was assessed in vitro by alkaline comet, DNA fragmentation and DAPI staining assays. TBHQ dose- and time-dependently decreased the growth of A549 and HUVEC cells. Flow cytometry analyses determined early and late apoptosis in the treated cells. Also, single strand DNA breaking has been observed through comet assay technique. In addition, morphology of DAPI stained cells and DNA fragmentation assay using gel electrophoresis showed clear fragmentation in the chromatin and DNA rings within the nucleus of cell's treated TBHQ. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：315 / 320

页数：6

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