Distribution of microorganisms in deep-sea hydrothermal vent chimneys investigated by whole-cell hybridization and enrichment culture of thermophilic subpopulations

被引:99
作者
Harmsen, HJM
Prieur, D
Jeanthon, C
机构
[1] CNRS UPR 9042,BIOL STN,F-29682 ROSCOFF,FRANCE
[2] UNIV PARIS 06,F-29682 ROSCOFF,FRANCE
[3] UNIV BRETAGNE OCCIDENTALE,UFR SCI & TECH,F-29285 BREST,FRANCE
关键词
D O I
10.1128/AEM.63.7.2876-2883.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The microbial community structure of hydrothermal vent chimneys was evaluated by the combined use of enrichment cultures and whole-cell hybridizations with fluorescently labeled 16S rRNA-based oligonucleotide probes, Chimneys were collected during the Microsmoke cruise on the Mid-Atlantic Ridge and were subsampled on board and stored under reduced conditions or fixed. For estimation of culturable thermophiles, selective media were inoculated by dilution series of the samples and incubated at 65, 80, and 95 degrees C, To analyze the microbial diversity of the samples, cells were extracted from the fixed chimney structure samples and hybridized with domain- and kingdom-specific probes, Quantification of the extracted cells was assessed by whole-cell hybridization on membrane filters, By both methods, the largest amounts of microorganisms were found in the upper and outer parts of the chimneys, although even the inner parts contained culturable and detectable amounts of cells, Different morphotypes of thermophilic and hyperthermophilic microorganisms were enriched and detected in samples, Our data clearly indicate that the morphological diversity observed by using whole-cell hybridization is much larger than that assessed by use of culture-based enrichments. This new approach, including culture-independent and -dependent methods to study hydrothermal vent chimneys, showed an uneven distribution of a diverse microbial community, Application of lower-level specific probes for known families and genera within each domain by our approach, will be useful to reveal the real extent and nature of the chimney microbial diversity and to support cultivation attempts.
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页码:2876 / 2883
页数:8
相关论文
共 42 条
[1]   FLUORESCENT-OLIGONUCLEOTIDE PROBING OF WHOLE CELLS FOR DETERMINATIVE, PHYLOGENETIC, AND ENVIRONMENTAL-STUDIES IN MICROBIOLOGY [J].
AMANN, RI ;
KRUMHOLZ, L ;
STAHL, DA .
JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :762-770
[2]   PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].
AMANN, RI ;
LUDWIG, W ;
SCHLEIFER, KH .
MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169
[3]   METHANOGENS - RE-EVALUATION OF A UNIQUE BIOLOGICAL GROUP [J].
BALCH, WE ;
FOX, GE ;
MAGRUM, LJ ;
WOESE, CR ;
WOLFE, RS .
MICROBIOLOGICAL REVIEWS, 1979, 43 (02) :260-296
[4]   NEW APPROACH TO CULTIVATION OF METHANOGENIC BACTERIA - 2-MERCAPTOETHANESULFONIC ACID (HS-COM)-DEPENDENT GROWTH OF METHANOBACTERIUM-RUMINANTIUM IN A PRESSURIZED ATMOSPHERE [J].
BALCH, WE ;
WOLFE, RS .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1976, 32 (06) :781-791
[5]   IDENTIFYING MEMBERS OF THE DOMAIN ARCHAEA WITH RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES [J].
BURGGRAF, S ;
MAYER, T ;
AMANN, R ;
SCHADHAUSER, S ;
WOESE, CR ;
STETTER, KO .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (09) :3112-3119
[6]   ARCHAEOGLOBUS-PROFUNDUS SP-NOV, REPRESENTS A NEW SPECIES WITHIN THE SULFATE-REDUCING ARCHAEBACTERIA [J].
BURGGRAF, S ;
JANNASCH, HW ;
NICOLAUS, B ;
STETTER, KO .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1990, 13 (01) :24-28
[7]   TRANSOVARIAL INHERITANCE OF ENDOSYMBIOTIC BACTERIA IN CLAMS INHABITING DEEP-SEA HYDROTHERMAL VENTS AND COLD SEEPS [J].
CARY, SC ;
GIOVANNONI, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5695-5699
[8]   BACTERIAL MATS FROM CRATER LAKE, OREGON AND THEIR RELATIONSHIP TO POSSIBLE DEEP-LAKE HYDROTHERMAL VENTING [J].
DYMOND, J ;
COLLIER, RW ;
WATWOOD, ME .
NATURE, 1989, 342 (6250) :673-675
[9]  
ERAUSO G, 1993, ARCH MICROBIOL, V160, P338
[10]   STAPHYLOTHERMUS-MARINUS SP-NOV REPRESENTS A NOVEL GENUS OF EXTREMELY THERMOPHILIC SUBMARINE HETEROTROPHIC ARCHAEBACTERIA GROWING UP TO 98-DEGREES-C [J].
FIALA, G ;
STETTER, KO ;
JANNASCH, HW ;
LANGWORTHY, TA ;
MADON, J .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1986, 8 (1-2) :106-113