Detection of starch granules in a living plant by optical second harmonic microscopy

被引：55

作者：

Mizutani, G

Sonoda, Y

Sano, H

Sakamoto, M

Takahashi, T

Ushioda, S

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Tatsunokuchi, Ishikawa 9231292, Japan

[2] Japan Sci & Technol Corp, PRESTO, Fields & React, Okayama 7000904, Japan

来源：

JOURNAL OF LUMINESCENCE | 2000年 / 87-9卷

关键词：

optical second harmonic microscopy; plant; starch;

D O I：

10.1016/S0022-2313(99)00428-7

中图分类号：

O43 [光学];

学科分类号：

070207 [光学]; 0803 [光学工程];

摘要：

We demonstrate a new method for detecting starch granules in a plant, based on optical second harmonic microscopy. In this method a plant is irradiated with infrared laser pulses and the image formed by scattered light at the second harmonic frequency is observed. A starch granule consists of well-ordered arrays of chiral glucose molecules and shows an intense optical second harmonic response. Thus they are selectively seen in the SH intensity image. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：824 / 826

页数：3

共 11 条

[1]

BACTERIORHODOPSIN ORIENTED IN POLYVINYL-ALCOHOL FILMS AS AN ERASABLE OPTICAL STORAGE MEDIUM [J].