Characterization of the Arabidopsis lysine-rich arabinogalactan-protein AtAGP17 mutant (rat1) that results in a decreased efficiency of agrobacterium transformation

被引:118
作者
Gaspar, YM
Nam, J
Schultz, CJ
Lee, LY
Gilson, PR
Gelvin, SB
Bacic, A [1 ]
机构
[1] Univ Melbourne, Sch Bot, Plant Cell Biol Res Ctr, Melbourne, Vic 3010, Australia
[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[3] Univ Adelaide, Waite Agr Res Inst, Sch Agr & Wine, Glen Osmond, SA 5064, Australia
关键词
D O I
10.1104/pp.104.045542
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. The Arabidopsis rat1 mutant, previously characterized as resistant to Agrobacterium tumefaciens root transformation, is due to a mutation in the gene for the Lys-rich AGP, AtAGP17. We show that the phenotype of rat1 correlates with down-regulation of AGP17 in the root as a result of a T-DNA insertion into the promoter of AGP17. Complementation of rat1 plants by a floral dip method with either the wild-type AGP17 gene or cDNA can restore the plant to a wild-type phenotype in several independent transformants. Based on changes in PR1 gene expression and a decrease in free salicylic acid levels upon Agrobacterium infection, we suggest mechanisms by which AGP17 allows Agrobacterium rapidly to reduce the systemic acquired resistance response during the infection process.
引用
收藏
页码:2162 / 2171
页数:10
相关论文
共 66 条
[1]   WAKs: cell wall-associated kinases linking the cytoplasm to the extracellular matrix [J].
Anderson, CM ;
Wagner, TA ;
Perret, M ;
He, ZH ;
He, DZ ;
Kohorn, BD .
PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) :197-206
[2]   NEW PLANT BINARY VECTORS WITH SELECTABLE MARKERS LOCATED PROXIMAL TO THE LEFT T-DNA BORDER [J].
BECKER, D ;
KEMPER, E ;
SCHELL, J ;
MASTERSON, R .
PLANT MOLECULAR BIOLOGY, 1992, 20 (06) :1195-1197
[3]   Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis [J].
Borner, GHH ;
Lilley, KS ;
Stevens, TJ ;
Dupree, P .
PLANT PHYSIOLOGY, 2003, 132 (02) :568-577
[4]   Prediction of glycosylphosphatidylinositol-anchored proteins in arabidopsis. A genomic analysis [J].
Borner, GHH ;
Sherrier, DJ ;
Stevens, TJ ;
Arkin, IT ;
Dupree, P .
PLANT PHYSIOLOGY, 2002, 129 (02) :486-499
[5]   Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana [J].
Cho, HT ;
Cosgrove, DJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (17) :9783-9788
[6]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[7]   A CENTRAL ROLE OF SALICYLIC-ACID IN PLANT-DISEASE RESISTANCE [J].
DELANEY, TP ;
UKNES, S ;
VERNOOIJ, B ;
FRIEDRICH, L ;
WEYMANN, K ;
NEGROTTO, D ;
GAFFNEY, T ;
GUTRELLA, M ;
KESSMANN, H ;
WARD, E ;
RYALS, J .
SCIENCE, 1994, 266 (5188) :1247-1250
[8]   Three unique mutants of Arabidopsis identify eds loci required for limiting growth of a biotrophic fungal pathogen [J].
Dewdney, J ;
Reuber, TL ;
Wildermuth, MC ;
Devoto, A ;
Cui, JP ;
Stutius, LM ;
Drummond, EP ;
Ausubel, FM .
PLANT JOURNAL, 2000, 24 (02) :205-218
[9]   Plant gene expression response to Agrobacterium tumefaciens [J].
Ditt, RF ;
Nester, EW ;
Comai, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (19) :10954-10959
[10]   Arabinogalactan-proteins: A class of extracellular matrix proteoglycans involved in plant growth and development [J].
Du, H ;
Clarke, AE ;
Bacic, A .
TRENDS IN CELL BIOLOGY, 1996, 6 (11) :411-414