Lactobacillus crispatus represses vaginolysin expression by BV associated Gardnerella vaginalis and reduces cell cytotoxicity

被引：26

作者：

Castro, Joana ^{[1
,2
]}

Martins, Ana Paula ^{[1
]}

Rodrigues, Maria Elisa ^{[1
]}

Cerca, Nuno ^{[1
]}

机构：

[1] Univ Minho, Lab Res Biofilms Rosario Oliveira LIBRO, Ctr Biol Engn CEB, Campus Gualtar, P-4710057 Braga, Portugal

[2] Univ Porto, ICBAS, Porto, Portugal

来源：

ANAEROBE | 2018年 / 50卷

关键词：

Bacterial vaginosis; G; vaginalis; L; crispatus; Vaginolysin; Sialidase; BACTERIAL-VAGINOSIS; BIOFILM FORMATION; GENITAL-TRACT; MODEL; MICROBIOME; ANAEROBES; ADHERENCE; SUGGESTS; WOMEN;

D O I：

10.1016/j.anaerobe.2018.01.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Using a chemically-defined medium simulating genital tract secretions, we have shown that preadhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis. This effect was associated to the expression of vaginolysin and was specific to L. crispatus interference, as other vaginal facultative anaerobes had no protective effect. (C) 2018 Elsevier Ltd. All rights reserved.

引用

收藏

页码：60 / 63

页数：4

相关论文

共 30 条

[1]

Gardnerella vaginalis Outcompetes 29 Other Bacterial Species Isolated From Patients With Bacterial Vaginosis, Using in an In Vitro Biofilm Formation Model [J].

Alves, Patricia ;

Castro, Joana ;

Sousa, Carmen ;

Cereija, Tatiana B. ;

Cerca, Nuno .

JOURNAL OF INFECTIOUS DISEASES, 2014, 210 (04) :593-596

[2]

Gardnerella vaginalis isolated from patients with bacterial vaginosis and from patients with healthy vaginal ecosystems [J].

Aroutcheva, AA ;

Simoes, JA ;

Behbakht, K ;

Faro, S .

CLINICAL INFECTIOUS DISEASES, 2001, 33 (07) :1022-1027

[3]

Identification, quantification and subtyping of Gardnerella vaginalis in noncultured clinical vaginal samples by quantitative PCR [J].

Balashov, Sergey V. ;

Mordechai, Eli ;

Adelson, Martin E. ;

Gygax, Scott E. .

JOURNAL OF MEDICAL MICROBIOLOGY, 2014, 63 :162-175

[4]

Interaction of vaginal Lactobacillus strains with HeLa cells plasma membrane [J].

Calonghi, N. ;

Parolin, C. ;

Sartor, G. ;

Verardi, L. ;

Giordani, B. ;

Frisco, G. ;

Marangoni, A. ;

Vitali, B. .

BENEFICIAL MICROBES, 2017, 8 (04) :625-633

[5]

Comparative transcriptomic analysis of Gardnerella vaginalis biofilms vs. planktonic cultures using RNA-seq [J].

Castro, Joana ;

Franca, Angela ;

Bradwell, Katie R. ;

Serrano, Myrna G. ;

Jefferson, Kimberly K. ;

Cerca, Nuno .

NPJ BIOFILMS AND MICROBIOMES, 2017, 3

[6]

BV and non-BV associated Gardnerella vaginalis establish similar synergistic interactions with other BV-associated microorganisms in dual-species biofilms [J].

Castro, Joana ;

Cerca, Nuno .

ANAEROBE, 2015, 36 :56-59

[7]

Using an in-vitro biofilm model to assess the virulence potential of Bacterial Vaginosis or non-Bacterial Vaginosis Gardnerella vaginalis isolates [J].

Castro, Joana ;

Alves, Patricia ;

Sousa, Carmen ;

Cereija, Tatiana ;

Franca, Angela ;

Jefferson, Kimberly K. ;

Cerca, Nuno .

SCIENTIFIC REPORTS, 2015, 5

[8]

Reciprocal Interference between Lactobacillus spp. and Gardnerella vaginalis on Initial Adherence to Epithelial Cells [J].

Castro, Joana ;

Henriques, Ana ;

Machado, Antonio ;

Henriques, Mariana ;

Jefferson, Kimberly K. ;

Cerca, Nuno .

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, 2013, 10 (09) :1193-1198

[9]

Bacterial vaginosis and adverse outcomes among full-term infants: a cohort study [J].

Dingens, Adam S. ;

Fairfortune, Tessa S. ;

Reed, Susan ;

Mitchell, Caroline .

BMC PREGNANCY AND CHILDBIRTH, 2016, 16

[10]

Optimizing a qPCR Gene Expression Quantification Assay for S. epidermidis Biofilms: A Comparison between Commercial Kits and a Customized Protocol [J].

Franca, Angela ;

Freitas, Ana I. ;

Henriques, Ana F. ;

Cerca, Nuno .

PLOS ONE, 2012, 7 (05)