Fluorescence assay for studying the ability of macrolides to induce production of ribosomal methylase

被引：13

作者：

Clarebout, G ^{[1
]}

Leclercq, R ^{[1
]}

机构：

[1] Univ Caen, Hop Cote Nacre, Microbiol Serv, UPRES EA 2128, F-14033 Caen, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2002年 / 46卷 / 07期

关键词：

D O I：

10.1128/AAC.46.7.2269-2272.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A screening assay to test the inducing capacity of macrolides by fusing the attenuator of the inducible erm(B) gene from Streptococcus pneumoniae HM28 with the gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer ketolides. Induction could be quantified by fluorometry.

引用

页码：2269 / 2272

页数：4

共 19 条

[1] Antibacterial activity of RU 64004 (HMR 3004), a novel ketolide derivative active against respiratory pathogens
Agouridas, C
Bonnefoy, A
Chantot, JF
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (10) : 2149 - 2158
[2] Ketolides lack inducibility properties of MLSB resistance phenotype
Bonnefoy, A
Girard, AM
Agouridas, C
Chantot, JF
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1997, 40 (01) : 85 - 90
[3] Ketolides - telithromycin, an example of a new class of antibacterial agents
Bryskier, A
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2000, 6 (12) : 661 - 669
[4] *COM ANT SOC FRANC, 1996, CLIN MICROBIOL INFEC, V2, P11
[5] β-keto-ester chemistry and ketolides.: Synthesis and antibacterial activity of 2-halogeno, 2-methyl and 2,3 enol-ether ketolides
Denis, A
Bretin, F
Fromentin, C
Bonnet, A
Piltan, G
Bonnefoy, A
Agouridas, C
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2000, 10 (17) : 2019 - 2022
[6] Synthesis and antibacterial activity of HMR 3647 a new ketolide highly potent against erythromycin-resistant and susceptible pathogens
Denis, A
Agouridas, C
Auger, JM
Benedetti, Y
Bonnefoy, A
Bretin, F
Chantot, JF
Dussarat, A
Fromentin, C
D'Ambrières, SG
Lachaud, S
Laurin, P
Le Martret, O
Loyau, V
Tessot, N
Pejac, JM
Perron, S
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1999, 9 (21) : 3075 - 3080
[7] Macrolide-ketolide inhibition of MLS-resistant ribosomes is improved by alternative drug interaction with domain II of 23S rRNA
Douthwaite, S
Hansen, LH
Mauvais, P
[J]. MOLECULAR MICROBIOLOGY, 2000, 36 (01) : 183 - 192
[8] BINDING OF NOVEL MACROLIDE STRUCTURES TO MACROLIDES-LINCOSAMIDES-STREPTOGRAMIN B-RESISTANT RIBOSOMES INHIBITS PROTEIN-SYNTHESIS AND BACTERIAL-GROWTH
GOLDMAN, RC
KADAM, SK
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (07) : 1058 - 1066
[9] Functional gene transfer from intracellular bacteria to mammalian cells
Grillot-Courvalin, C
Goussard, S
Huetz, F
Ojcius, DM
Courvalin, P
[J]. NATURE BIOTECHNOLOGY, 1998, 16 (09) : 862 - 866
[10] A COMPLEX ATTENUATOR REGULATES INDUCIBLE RESISTANCE TO MACROLIDES, LINCOSAMIDES, AND STREPTOGRAMIN TYPE-B ANTIBIOTICS IN STREPTOCOCCUS-SANGUIS
HORINOUCHI, S
BYEON, WH
WEISBLUM, B
[J]. JOURNAL OF BACTERIOLOGY, 1983, 154 (03) : 1252 - 1262

← 1 2 →