A polymorphism in the mouse Crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay

被引：23

作者：

Hallensleben, W

Biro, L

Sauder, C

Hausmann, J

Asensio, VC

Campbell, IL

Staeheli, P

机构：

[1] Univ Freiburg, Inst Med Mikrobiol & Hyg, Abt Virol, D-79008 Freiburg, Germany

[2] Scripps Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2000年 / 234卷 / 1-2期

关键词：

ribonuclease protection assay; CXC chemokine; Crg-2/IP-10 gene polymorphism;

D O I：

10.1016/S0022-1759(99)00197-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：149 / 151

页数：3