A polymorphism in the mouse Crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay

被引:23
作者
Hallensleben, W
Biro, L
Sauder, C
Hausmann, J
Asensio, VC
Campbell, IL
Staeheli, P
机构
[1] Univ Freiburg, Inst Med Mikrobiol & Hyg, Abt Virol, D-79008 Freiburg, Germany
[2] Scripps Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA
关键词
ribonuclease protection assay; CXC chemokine; Crg-2/IP-10 gene polymorphism;
D O I
10.1016/S0022-1759(99)00197-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
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页码:149 / 151
页数:3
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