共 1 条
A polymorphism in the mouse Crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay
被引:23
作者:
Hallensleben, W
Biro, L
Sauder, C
Hausmann, J
Asensio, VC
Campbell, IL
Staeheli, P
机构:
[1] Univ Freiburg, Inst Med Mikrobiol & Hyg, Abt Virol, D-79008 Freiburg, Germany
[2] Scripps Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA
关键词:
ribonuclease protection assay;
CXC chemokine;
Crg-2/IP-10 gene polymorphism;
D O I:
10.1016/S0022-1759(99)00197-0
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:149 / 151
页数:3
相关论文