The reduction of the Rieske iron-sulfur cluster in naphthalene dioxygenase by X-rays

被引：33

作者：

Karlsson, A

Parales, JV

Parales, RE

Gibson, DT

Eklund, H

Ramaswamy, S

机构：

[1] Swedish Univ Agr Sci, Dept Mol Biol, Ctr Biomed, S-75124 Uppsala, Sweden

[2] Univ Iowa, Dept Microbiol, Iowa City, IA 52252 USA

[3] Univ Iowa, Ctr Biocatalysis & Bioproc, Iowa City, IA 52252 USA

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2000年 / 78卷 / 01期

关键词：

D O I：

10.1016/S0162-0134(99)00213-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Naphthalene 1,2 dioxygenase (NDO) displays characteristic UV-Vis spectra depending on the oxidation state of the Rieske center. Investigations on crystals of NDO grown for X-ray diffraction experiments showed spectra characteristic of the oxidized form. Crystals reduced in an anaerobic glovebox using sodium-dithionite-showed a characteristic reduced spectrum. Spectra of crystals (cooled to 100 K) after being exposed to X-rays for data collection showed spectra corresponding to a reduced Rieske iron center, demonstrating the ability of X-rays to change the oxidation state of the Rieske iron-sulfur cluster in NDO. (C) 2000 Elsevier Science Inc. All rights reserved.

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页码：83 / 87

页数：5

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