Comparison of the real-time PCR method and the gen-probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens

被引:45
作者
Lemaître, N
Armand, S
Vachée, A
Capilliez, O
Dumoulin, C
Courcol, RJ
机构
[1] CHRU Lille, Lab Bacteriol Hyg, F-59037 Lille, France
[2] Hop Roubaix, Lab Biol Polyvalente, Roubaix, France
关键词
D O I
10.1128/JCM.42.9.4307-4309.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Real-time PCR was compared to Amplified Mycobacterium tuberculosis Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDH were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII.
引用
收藏
页码:4307 / 4309
页数:3
相关论文
共 13 条
[1]   Rapid and specific detection of Mycobacterium tuberculosis by using the smart cycler instrument and a specific fluorogenic probe [J].
Cleary, TJ ;
Roudel, G ;
Casillas, O ;
Miller, N .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4783-4786
[2]   Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis [J].
Desjardin, LE ;
Chen, Y ;
Perkins, MD ;
Teixeira, L ;
Cave, MD ;
Eisenach, KD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :1964-1968
[3]   Comparative evaluation of initial and new versions of the Gen-Probe amplified Mycobacterium Tuberculosis Direct Test for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens [J].
Gamboa, F ;
Fernandez, G ;
Padilla, E ;
Manterola, JM ;
Lonca, J ;
Cardona, PJ ;
Matas, L ;
Ausina, V .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (03) :684-689
[4]   Evaluation of a new commercial assay for diagnosis of pulmonary and nonpulmonary tuberculosis [J].
Johansen, IS ;
Thomsen, VO ;
Johansen, A ;
Andersen, P ;
Lundgren, B .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2002, 21 (06) :455-460
[5]   Clinical utility of an amplification test based on ligase chain reaction in pulmonary tuberculosis [J].
Jouveshomme, S ;
Cambau, E ;
Trystram, D ;
Szpytma, M ;
Sougakoff, W ;
Derenne, JP ;
Grosset, J .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1998, 158 (04) :1096-1101
[6]   Use of equivocal zone in interpretation of results of the amplified mycobacterium tuberculosis direct test for diagnosis of tuberculosis [J].
Kerleguer, A ;
Koeck, JL ;
Fabre, M ;
Gérôme, P ;
Teyssou, R ;
Hervé, V .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (04) :1783-1784
[7]  
Metchock BG., 1999, Manual of clinical microbiologyx. Dans: Mycobacterium, V7th ed, P399
[8]   Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct test by using respiratory and nonrespiratory specimens in a tertiary care center laboratory [J].
O'Sullivan, CE ;
Miller, DR ;
Schneider, PS ;
Roberts, GD .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (05) :1723-1727
[9]   Performance assessment of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex from respiratory and extrapulmonary specimens [J].
Piersimoni, C ;
Scarparo, C ;
Piccoli, P ;
Rigon, A ;
Ruggiero, G ;
Nista, D ;
Bornigia, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4138-4142
[10]   Comparative evaluation of the new gen-probe Mycobacterium tuberculosis amplified direct test and the semiautomated Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in respiratory and extrapulmonary specimens [J].
Piersimoni, C ;
Callegaro, A ;
Scarparo, C ;
Penati, V ;
Nista, D ;
Bornigia, S ;
Lacchini, C ;
Scagnelli, M ;
Santini, G ;
De Sio, G .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (12) :3601-3604