Binding capacities for Thermomonospora fusca E-3, E-4 and E-5, the E-3 binding domain, and Trichoderma reesei CBHI on Avicel and bacterial microcrystalline cellulose

被引:37
作者
Bothwell, MK
Daughhetee, SD
Chaua, GY
Wilson, DB
Walker, LP
机构
[1] CORNELL UNIV, BIOCHEM MOL & CELL BIOL SECT, ITHACA, NY 14853 USA
[2] OREGON STATE UNIV, DEPT BIORESOURCE ENGN, CORVALLIS, OR 97331 USA
关键词
Langmuir isotherm; binding kinetics; cellulases; porosity; morphology; Thermomonospora fusca; Trichoderma reesei;
D O I
10.1016/S0960-8524(96)00179-4
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Equilibrium binding of Thermomonospora fusca E-3, E-4 and E-5, the E-3 binding domain (CBDE3), and Trichoderma reesei CBHI on Avicel PH102 and bacterial microcrystalline cellulose (BMCC) was studied. The maximum adsorption levels, E-b,E-m, for all four cellulases and the binding domain were 9-30 times higher on BMCC than on Avicel. The association constants for the individual cellulases were dependent upon the substrate; however no obvious patterns were noted. A comparison of the T. fusca EbmS showed a decreasing power function relationship between molecular weight and maximum adsorption levels. This was particularly true for the cellulases binding on Avicel. The T. fusca binding results strongly suggest that binding capacity is a function of the cellulase size and the pore structure of the cellulose. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:169 / 178
页数:10
相关论文
共 55 条
[1]   ADSORPTION AND KINETIC-BEHAVIOR OF PURIFIED ENDOGLUCANASES AND EXOGLUCANASES FROM TRICHODERMA-VIRIDE [J].
BELDMAN, G ;
VORAGEN, AGJ ;
ROMBOUTS, FM ;
SEARLEVANLEEUWEN, MF ;
PILNIK, W .
BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (02) :251-257
[2]  
BISARIA VS, 1978, BIOCONVERSION CELLUL, P155
[3]   EVALUATION OF PARAMETER-ESTIMATION METHODS FOR ESTIMATING CELLULASE BINDING CONSTANTS [J].
BOTHWELL, MK ;
WALKER, LP .
BIORESOURCE TECHNOLOGY, 1995, 53 (01) :21-29
[4]   A NEW METHOD OF CONSTRAINED OPTIMIZATION AND A COMPARISON WITH OTHER METHODS [J].
BOX, MJ .
COMPUTER JOURNAL, 1965, 8 (01) :42-52
[5]  
CHEN C M, 1987, Bio-Technology (New York), V5, P274
[6]   NUCLEOTIDE-SEQUENCE OF THE ENDOGLUCANASE-C GENE (CENC) OF CELLULOMONAS-FIMI, ITS HIGH-LEVEL EXPRESSION IN ESCHERICHIA-COLI, AND CHARACTERIZATION OF ITS PRODUCTS [J].
COUTINHO, JB ;
MOSER, B ;
KILBURN, DG ;
WARREN, RAJ ;
MILLER, RC .
MOLECULAR MICROBIOLOGY, 1991, 5 (05) :1221-1233
[7]   KINETIC-STUDIES OF ENZYMATIC-HYDROLYSIS OF INSOLUBLE CELLULOSE - DERIVATION OF A MECHANISTIC KINETIC-MODEL [J].
FAN, LT ;
LEE, YH .
BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (11) :2707-2733
[8]  
GEBLER J, 1992, J BIOL CHEM, V267, P12559
[9]  
GILKES NR, 1992, J BIOL CHEM, V267, P6743
[10]  
GILKES NR, 1988, J BIOL CHEM, V263, P10401