Scrapie-infected cells, isolated prions, and recombinant prion protein: A comparative study

被引：18

作者：

Kneipp, J

Miller, LM

Spassov, S

Sokolowski, F

Lasch, P

Beekes, M

Naumann, D

机构：

[1] Robert Koch Inst, D-13353 Berlin, Germany

[2] Brookhaven Natl Lab, Natl Synchrotron Light Source, Upton, NY 11973 USA

来源：

BIOPOLYMERS | 2004年 / 74卷 / 1-2期

关键词：

SHaPrP(90-232); scrapie; 263K; prion; transmissible spongiform encephalopathies; Syrian hamster; FTIR; dorsal root ganglia;

D O I：

10.1002/bip.20064

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fourier-transform infrared microscopic spectra of scrapie-infected nervous tissue measured at high spatial resolution (similar to6 mum) were compared with those obtained from the purified, pardly proteinase K digested scrapie isoform of the prion protein isolated from nervous tissue of hamsters infected with the same scrapie strain (263K) to elucidate similarities/dissimilarities between prion structure investigated in situ and ex vivo. A further comparison is drawn to the recombinant Syrian hamster prion protein SHaPrP(90-232) after in vitro conformational transition front the predominantly alpha-helical isoform to beta-sheet-rich structures. It is shown that prion protein structure can be investigated within tissue and that detectability of regions with elevated beta-sheet content as observed in microspectra of prion-infected tissue strongly depends on spatial resolution of the experiment. (C) 2004 Wiley Periodicals, Inc.

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收藏

页码：163 / 167

页数：5

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