p300 collaborates with Sp1 and Sp3 in p21waf1/cip1 promoter activation induced by histone deacetylase inhibitor

被引:150
作者
Xiao, HY
Hasegawa, T
Isobe, K
机构
[1] Natl INst Longev Sci, Dept Basic Gerontol, Aichi 4748522, Japan
[2] W China Univ Med Sci, Canc Res Inst, Chengdu 610041, Peoples R China
关键词
D O I
10.1074/jbc.275.2.1371
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have reported that histone acetylation induced by trichostatin A (TSA) promotes p21(waf1/cip1) (p21) expression, the GC-box located just upstream of TATA box was responsible for TSA-induced promoter activation, and both Spl and Sp3 were the working activator of this GC-box, To understand the molecular pathway from histone acetylation to this Spl family factors-mediated promoter activation, we investigated the function of p300, one of the histone acetyltransferase, in the present work. The evidence supporting the linkage between p300 and TSA-induced p21 promoter activation were realized from the following findings: 1) cotransfection of p300 elevated pal promoter activity, and this elevation was dependent on TSA-responsive GC-box; 2) TSA-induced promoter activation was blocked by the introduction of p300 dominant-negative mutant into cells; and 3) Sp1- or Sp3-mediated activation was also suppressed by this p300 dominant-negative mutant. Our data also suggested that p300 collaborates with Spl in a way which is different from that when p300 collaborates with p53 in p21 transcription.
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页码:1371 / 1376
页数:6
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