RIO1, an extraordinary novel protein kinase

被引：41

作者：

Angermayr, M ^{[1
]}

Bandlow, W ^{[1
]}

机构：

[1] Univ Munich, Bereich Genet, Dept Biol 1, D-80638 Munich, Germany

来源：

FEBS LETTERS | 2002年 / 524卷 / 1-3期

关键词：

protein kinase; secondary structure alignment; Rio1 kinase signature; Saccharomyces cerevisiae;

D O I：

10.1016/S0014-5793(02)02993-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RIOI and Rio-related proteins display little similarity of primary sequence with conventional protein kinases. Based on secondary structure alignments, we show that it contains the domain structure (subdomains I-XI) and conserved secondary structure elements found in conventional protein kinases. We show that recombinant wild-type Rio1p isolated from Escherichia coli displays kinase activity which depends on autophosphorylation and magnesium or manganese as ATP-activating ions. An initial biochemical characterization of Rio1p is presented. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：31 / 36

页数：6

共 22 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] Isolation of the Aspergillus nidulans sudD gene and its human homologue [J].

Anaya, P ;

Evans, SC ;

Dai, CP ;

Lozano, G ;

May, GS .

GENE, 1998, 211 (02) :323-329

[3] Yeast Rio1p is the founding member of a novel subfamily of protein serine kinases involved in the control of cell cycle progression [J].

Angermayr, M ;

Roidl, A ;

Bandlow, W .

MOLECULAR MICROBIOLOGY, 2002, 44 (02) :309-324

[4] The type of basal promoter determines the regulated or constitutive mode of transcription in the common control region of the yeast gene pair GCY1/RIO1 [J].

Angermayr, M ;

Bandlow, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (50) :31630-31635

[5] The PROSITE database, its status in 1997 [J].

Bairoch, A ;

Bucher, P ;

Hofmann, K .

NUCLEIC ACIDS RESEARCH, 1997, 25 (01) :217-221

[6] PHOSPHOTRANSFERASE AND SUBSTRATE BINDING MECHANISM OF THE CAMP-DEPENDENT PROTEIN-KINASE CATALYTIC SUBUNIT FROM PORCINE HEART AS DEDUCED FROM THE 2.0 ANGSTROM STRUCTURE OF THE COMPLEX WITH MN2+ ADENYLYL IMIDODIPHOSPHATE AND INHIBITOR PEPTIDE PKI(5-24) [J].

BOSSEMEYER, D ;

ENGH, RA ;

KINZEL, V ;

PONSTINGL, H ;

HUBER, R .

EMBO JOURNAL, 1993, 12 (03) :849-859

[7]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[8] INCREASED SOLUBILITY OF TRIMETHOPRIM-RESISTANT TYPE S1 DHFR FROM STAPHYLOCOCCUS-AUREUS IN ESCHERICHIA-COLI-CELLS OVERPRODUCING THE CHAPERONINS GROEL AND GROES [J].

DALE, GE ;

SCHONFELD, HJ ;

LANGEN, H ;

STIEGER, M .

PROTEIN ENGINEERING, 1994, 7 (07) :925-931

[9] Life with 6000 genes [J].

Goffeau, A ;

Barrell, BG ;

Bussey, H ;

Davis, RW ;

Dujon, B ;

Feldmann, H ;

Galibert, F ;

Hoheisel, JD ;

Jacq, C ;

Johnston, M ;

Louis, EJ ;

Mewes, HW ;

Murakami, Y ;

Philippsen, P ;

Tettelin, H ;

Oliver, SG .

SCIENCE, 1996, 274 (5287) :546-&

[10] THE PROTEIN-KINASE FAMILY - CONSERVED FEATURES AND DEDUCED PHYLOGENY OF THE CATALYTIC DOMAINS [J].

HANKS, SK ;

QUINN, AM ;

HUNTER, T .

SCIENCE, 1988, 241 (4861) :42-52

← 1 2 3 →