Possible involvement of orexin in the stress reaction in rats

被引：234

作者：

Ida, T

Nakahara, K

Murakami, T

Hanada, R

Nakazato, M

Murakami, N ^{[1
]}

机构：

[1] Miyazaki Univ, Fac Agr, Dept Vet Physiol, Miyazaki 8892155, Japan

[2] Miyazaki Univ, Fac Agr, Dept Vet Anat, Miyazaki 8892155, Japan

[3] Oita Med Univ, Sch Med, Dept Internal Med 1, Oita 8795593, Japan

[4] Miyazaki Med Coll, Dept Internal Med 3, Kiyotake, Miyazaki 8891692, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 270卷 / 01期

关键词：

D O I：

10.1006/bbrc.2000.2412

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined whether corticotropin-releasing factor (CRF) was involved in orexin-induced grooming and face-washing behaviors, and whether orexin was involved in the stress reaction. Administration of alpha-helical CRF, CRF antagonist, alone had no behavioral effect, but it blocked the orexin-induced grooming and face-washing behaviors in rats. The level of corticosterone increased in a dose-dependent manner 15 min after icy injection of orexin, and it remained high for at least 60 min. In a-month-old rats, 1 h of immobilization stress increased orexin mRNA levels, but not the melanin-concentrating hormone (MCH) mRNG in the lateral hypothalamic area (LHA). In 6-month-old rats, 30 min of cold stress increased the expression of orexin mRNA in the LHA. Unlike in the 2-month-old rats, immobilization stress did not change orexin mRNA expression in g-month-old rats. These results suggest that CRF is involved in orexin-induced behaviors, and that orexin may play an important role in some stress reactions. (C) 2000 Academic Press.

引用

页码：318 / 323

页数：6

共 44 条

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