Analysis of membrane protein interactions using yeast-based technologies

被引：62

作者：

Stagljar, I

Fields, S

机构：

[1] Univ Zurich, Inst Vet Biochem & Mol Biol, CH-8057 Zurich, Switzerland

[2] Univ Washington, Howard Hughes Med Inst, Dept Genome Sci, Seattle, WA 98195 USA

[3] Univ Washington, Howard Hughes Med Inst, Dept Med, Seattle, WA 98195 USA

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2002年 / 27卷 / 11期

关键词：

D O I：

10.1016/S0968-0004(02)02197-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins associated with membranes total approximately a third of all proteins in a typical eukaryotic cell. However, the analysis of interactions between membrane proteins is difficult because of the hydrophobic nature of these proteins, and conventional biochemical and genetic assays are often of limited use. We summarize here recent yeast-based interaction technologies that can be applied to membrane proteins.

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页码：559 / 563

页数：5

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