Retrotransposition of limited deletion type of intracisternal A-particle elements in the myeloid leukemia clls of C3H/He mice

被引:22
作者
Ishihara, H
Tanaka, I
Wan, H
Nojima, K
Yoshida, K
机构
[1] Natl Inst Radiol Sci, Redox Regulat Res Grp, Inage Ku, Chiba 2638555, Japan
[2] Natl Inst Radiol Sci, Int Space Radiat Lab, Chiba 2638555, Japan
[3] Natl Inst Radiol Sci, Radiat Hazard Res Grp, Radiat Safety Res Ctr, Chiba 2638555, Japan
关键词
genomic IAP element; physical map of IAP element; full-length IAP element; integration; target duplication;
D O I
10.1269/jrr.45.25
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The murine genome has about 1,000 copies of DNA elements for the intracisternal A-particle (IAP) that resembles a retrovirus. We previously reported that the genomic DNA of the cells from radiation-induced acute myeloid leukemia (AML) lines derived from C3H/He inbred mice was frequently rearranged by the integration of the IAP element. In this study, 8 IAP elements from the characteristic integration sites in 6 cell lines of radiation-induced AML from different mice were characterized and compared in structure with 114 IAP elements isolated from the normal C3H/He genome. One of the 8 elements was a full-length type I IAP, and 7 were of type-IDeltaI with a common deletion site. Although the type IDeltaI form is a minor population accounting for about 6% of total genomic IAP elements, it is predominantly retrotransposed in the AML cells from different C3H/He mice. This indicates that limited populations of the IAP elements contribute to the unique retrotransposition in AML cells.
引用
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页码:25 / 32
页数:8
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