Lens epithelium-derived growth factor: Effects on growth and survival of lens epithelial cells, keratinocytes, and fibroblasts

被引：129

作者：

Singh, DP

Ohguro, N

Kikuchi, T

Sueno, T

Reddy, VN

Yuge, K

Chylack, LT

Shinohara, T ^{[1
]}

机构：

[1] Brigham & Womens Hosp, Ctr Ophthalm Res, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Ophthalmol, Boston, MA 02115 USA

[3] Oakland Univ, Eye Res Inst, Rochester, MI USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 267卷 / 01期

关键词：

autoantibody; autocrine; age-related cataract; lens epithelium-derived growth factor (LEDGF); survival factor; trafficking;

D O I：

10.1006/bbrc.1999.1979

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a clone encoding a protein from a human lens epithelial cell (LEC) cDNA library with antibody (Ab) from a cataract patient and named it "lens epithelium-derived growth factor" (LEDGF). LEDGF is found to be identical to p75, a coactivator of both transcription (1) and pre-mRNA splicing (2), In serum-free medium LEDGF stimulated growth of LECs, cos7 cells, skin fibroblasts, and keratinocytes, and prolonged cell survival. Without LEDGF, the aforementioned cells did not survive, Also in serum-free medium, Ab to LEDGF neutralizing LEDGF blocked cell growth and caused cell death. Thus, LEDGF, a regulatory factor, may play an important role for growth and survival of a wide range of cell types. (C) 2000 Academic Press.

引用

页码：373 / 381

页数：9