Tissue responses against immunoisolating alginate-PLL capsules in the immediate posttransplant period

被引:55
作者
de Vos, P
van Hoogmoed, CG
de Haan, BJ
Busscher, HJ
机构
[1] Univ Groningen, Dept Pathol, Sect Med Biol, NL-9700 RB Groningen, Netherlands
[2] Univ Groningen, Dept Lab Med, Sect Med Biol, NL-9700 RB Groningen, Netherlands
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 2002年 / 62卷 / 03期
关键词
alginate; poly-L-lysine; encapsulation; X-ray photoelectron spectroscopy; protein adsorption;
D O I
10.1002/jbm.10345
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Alginate-polylysine (PLL) capsules are commonly applied for immunoisolation of living cells for the treatment of a wide variety of diseases. Large-scale application of the technique, however, is hampered by insufficient biocompatibility of the capsules with failure of the grafts as a consequence. Most studies addressing biocompatibility issues of alginate-PLL capsules have focused on the degree of overgrowth on the capsules after graft failure and not on the reaction against the capsules in the immediate posttrans-plant period. Therefore, capsules were implanted in the peritoneal cavity of rats and retrieved 1, 5, and 7 days later for histological examination and X-ray photoelectron spectroscopy analysis for evaluation of chemical changes at the capsule surface. After implantation, the nitrogen signal increased from 5% on day 0, to 8.6% on day 7, illustrating protein adsorption on the capsule's surface. This increase in protein content of the membrane was accompanied by an increase in the percentage of overgrown capsules from 0.5 +/- 0.3% on day 1 to 3.3 +/- 1.6% on day 7. The cellular overgrowth was composed of monocytes/macrophages, granulocytes, fibroblasts, erythrocytes, multinucleated giant cells, and basophils. This overgrowth was not statical as generally assumed but rather dynamic as illustrated by our observation that at day 1 after implantation we mainly found monocytes/macrophages and granulocytes that on later time points were substituted by fibroblasts. As the inflammatory reaction predictably interfere with survival of encapsulated cells, efforts should be made to suppress activities or recruitment of inflammatory cells. These efforts may be temporary rather than permanent because most inflammatory cells have disappeared after 2 weeks of implantation. (C) 2002 Wiley Periodicals, Inc.
引用
收藏
页码:430 / 437
页数:8
相关论文
共 69 条
[1]   FUNCTIONAL RECOVERY IN HEMIPARKINSONIAN PRIMATES TRANSPLANTED WITH POLYMER-ENCAPSULATED PC12 CELLS [J].
AEBISCHER, P ;
GODDARD, M ;
SIGNORE, AP ;
TIMPSON, RL .
EXPERIMENTAL NEUROLOGY, 1994, 126 (02) :151-158
[2]  
AEBISCHER P, 1986, T AM SOC ART INT ORG, V32, P134
[3]   CORRECTION OF THE GROWTH DEFECT IN DWARF MICE WITH NONAUTOLOGOUS MICROENCAPSULATED MYOBLASTS - AN ALTERNATE APPROACH TO SOMATIC GENE-THERAPY [J].
ALHENDY, A ;
HORTELANO, G ;
TANNENBAUM, GS ;
CHANG, PL .
HUMAN GENE THERAPY, 1995, 6 (02) :165-175
[4]  
AROCK M, 1993, J IMMUNOL, V151, P1441
[5]   Host response to tissue engineered devices [J].
Babensee, JE ;
Anderson, JM ;
McIntire, LV ;
Mikos, AG .
ADVANCED DRUG DELIVERY REVIEWS, 1998, 33 (1-2) :111-139
[6]   UTILIZATION OF ACTIVATED U937 MONOCYTIC CELLS AS A MODEL TO EVALUATE BIOCOMPATIBILITY AND BIODEGRADATION OF SYNTHETIC CALCIUM-PHOSPHATE [J].
BLOTTIERE, HM ;
DACULSI, G ;
ANEGON, I ;
POUEZAT, JA ;
NELSON, PN ;
PASSUTI, N .
BIOMATERIALS, 1995, 16 (06) :497-503
[7]   HUMAN PERIPHERAL-BLOOD BASOPHILS PRIMED BY INTERLEUKIN-3 (IL-3) PRODUCE IL-4 IN RESPONSE TO IMMUNOGLOBULIN-E-RECEPTOR STIMULATION [J].
BRUNNER, T ;
HEUSSER, CH ;
DAHINDEN, CA .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (03) :605-611
[8]   DELIVERY OF RECOMBINANT GENE-PRODUCTS WITH MICROENCAPSULATED CELLS IN-VIVO [J].
CHANG, PL ;
SHEN, N ;
WESTCOTT, AJ .
HUMAN GENE THERAPY, 1993, 4 (04) :433-440
[9]   TISSUE ENGINEERING OF A BIOARTIFICIAL KIDNEY [J].
CIESLINSKI, DA ;
HUMES, HD .
BIOTECHNOLOGY AND BIOENGINEERING, 1994, 43 (07) :678-681
[10]   Why do microencapsulated islet grafts fail in the absence of fibrotic overgrowth? [J].
De Vos, P ;
Van Straaten, JFM ;
Nieuwenhuizen, AG ;
de Groot, M ;
Ploeg, RJ ;
De Haan, PJ ;
Van Schilfgaarde, R .
DIABETES, 1999, 48 (07) :1381-1388