Human Skin 3D Bioprinting Using Scaffold-Free Approach

被引：257

作者：

Pourchet, Lea J. ^{[1
]}

Thepot, Amelie ^{[2
]}

Albouy, Marion ^{[2
]}

Courtial, Edwin J. ^{[1
]}

Boher, Aurelie ^{[2
]}

Blum, Loic J. ^{[1
]}

Marquette, Christophe A. ^{[1
]}

机构：

[1] Univ Lyon1, CPE Lyon,UMR 5246, CNRS,3d FAB, INSA,ICBMS, 43,Bd 11 novembre 1918, F-69622 Villeurbanne, France

[2] Hop Edouard Herriot, LabSkin Creat, 5 Place dArsonval,Batiment 5, F-69437 Lyon, France

来源：

ADVANCED HEALTHCARE MATERIALS | 2017年 / 6卷 / 04期

关键词：

FABRICATION; KERATINOCYTES; FIBROBLASTS; EQUIVALENT; ALGINATE; CULTURE; MODEL;

D O I：

10.1002/adhm.201601101

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Organ in vitro synthesis is one of the last bottlenecks between tissue engineering and transplantation of synthetic organs. Bioprinting has proven its capacity to produce 3D objects composed of living cells but highly organized tissues such as full thickness skin (dermis + epidermis) are rarely attained. The focus of the present study is to demonstrate the capability of a newly developed ink formulation and the use of an open source printer, for the production of a really complete skin model. Proofs are given through immunostaining and electronic microscopy that the bioprinted skin presents all characteristics of human skin, both at the molecular and macromolecular level. Finally, the printability of large skin objects is demonstrated with the printing of an adult-size ear.

引用

页数：8

共 19 条

[1]

[Anonymous], 2013, MERCK INDEX

[2]

Auger FA, 2004, BIOTECHNOL APPL BIOC, V39, P263

[3]

COLLAGEN-SYNTHESIS BY FIBROBLASTS CULTURED WITHIN A COLLAGEN SPONGE [J].