Identification and Purification of Human Induced Pluripotent Stem Cell-Derived Atrial-Like Cardiomyocytes Based on Sarcolipin Expression

被引：35

作者：

Josowitz, Rebecca ^{[1
]}

Lu, Jia ^{[2
]}

Falce, Christine ^{[3
]}

D'Souza, Sunita L. ^{[4
]}

Wu, Meng ^{[1
,5
]}

Cohen, Ninette ^{[5
]}

Dubois, Nicole C. ^{[1
,4
]}

Zhao, Yong ^{[1
,5
]}

Sobie, Eric A. ^{[3
]}

Fishman, Glenn I. ^{[2
,6
]}

Gelb, Bruce D. ^{[1
,5
,7
]}

机构：

[1] Icahn Sch Med Mt Sinai, Mindich Child Hlth & Dev Inst, New York, NY 10029 USA

[2] NYU, Sch Med, Leon H Charney Div Cardiol, New York, NY USA

[3] Icahn Sch Med Mt Sinai, Dept Pharmacol & Syst Therapeut, New York, NY 10029 USA

[4] Icahn Sch Med Mt Sinai, Dept Dev & Regenerat Biol, New York, NY 10029 USA

[5] Icahn Sch Med Mt Sinai, Dept Genet & Genom Sci, New York, NY 10029 USA

[6] NYU, Sch Med, Dept Med, New York, NY USA

[7] Icahn Sch Med Mt Sinai, New York, NY 10029 USA

来源：

PLOS ONE | 2014年 / 9卷 / 07期

基金：

美国国家卫生研究院;

关键词：

MATURATION; PHARMACOLOGY; SELECTION; PROTEIN; MUSCLE;

D O I：

10.1371/journal.pone.0101316

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The use of human stem cell-derived cardiomyocytes to study atrial biology and disease has been restricted by the lack of a reliable method for stem cell-derived atrial cell labeling and purification. The goal of this study was to generate an atrial-specific reporter construct to identify and purify human stem cell-derived atrial-like cardiomyocytes. We have created a bacterial artificial chromosome (BAC) reporter construct in which fluorescence is driven by expression of the atrial-specific gene sarcolipin (SLN). When purified using flow cytometry, cells with high fluorescence specifically express atrial genes and display functional calcium handling and electrophysiological properties consistent with atrial cardiomyocytes. Our data indicate that SLN can be used as a marker to successfully monitor and isolate hiPSC-derived atrial-like cardiomyocytes. These purified cells may find many applications, including in the study of atrial-specific pathologies and chamber-specific lineage development.

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