Clonal distribution of natural competence in Actinobacillus actinomycetemcomitans

被引：33

作者：

Fujise, O

Lakio, L

Wang, Y

Asikainen, S

Chen, C

机构：

[1] Univ So Calif, Sch Dent, Div Primary Oral Hlth Care, Los Angeles, CA 90089 USA

[2] Univ Helsinki, Inst Dent, Helsinki, Finland

[3] Umea Univ, Div Oral Microbiol, Umea, Sweden

[4] Kyushu Univ, Fac Dent Sci, Div Oral Rehabil, Fukuoka 812, Japan

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 2004年 / 19卷 / 05期

关键词：

clonal analysis; natural competence; natural transformation; periodontal disease;

D O I：

10.1111/j.1399-302x.2004.00157.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 [口腔医学];

摘要：

The competence for natural transformation was investigated in 67 Actinobacillus actinomycetemcomitans strains. The transformation assays were performed with both cloned DNA fragments and chromosomal markers of A. actinomycetemcomitans. Competence was found in 12 of 18 serotype a strains, 0 of 21 serotype b strains, 0 of 14 serotype c strains, 3 of 6 serotype d strains, 3 of 4 serotype e strains, 0 of 3 serotype f strains, and 0 of 1 nonserotypeable strain. The transformation frequencies varied from 5 x 10(-3) to 4 x 10(-6) (median 1.5 x 10(-4)). The distribution pattern of natural competence is concordant with the major clonal lineages of A. actinomycetemcomitans. Serotype a strains are predominantly competent for transformation, while serotypes b and c strains are apparently non-competent.

引用

页码：340 / 342

页数：3

共 30 条

[1]

Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions [J].