Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens

被引：23

作者：

Selan, L

Passariello, C

Rizzo, L

Varesi, P

Speziale, F

Renzini, G

Thaller, MC

Fiorani, P

Rossolini, GM ^{[1
]}

机构：

[1] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, Siena, Italy

[2] Univ Roma Tor Vergata, Dipartimento Biol, I-00173 Rome, Italy

[3] Univ Roma La Sapienza, Dipartimento Sci Sanita Pubbl, Rome, Italy

[4] Univ Roma La Sapienza, Dipartimento Chirurg Vasc, Rome, Italy

来源：

LANCET | 2002年 / 359卷 / 9324期

关键词：

D O I：

10.1016/S0140-6736(02)09086-4

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Late-onset Infections of synthetic vascular grafts (LO-SVGIs) are generally caused by staphylococcl that produce a slime polysaccharide and grow as a biofilm on the graft surface. We developed an ELISA to detect serum antibodies against staphylococcal slime polysaccharide antigens (SSPA). Patients with an ongoing staphylococcal LO-SVGI had greater titres of IgM antibodies against SSPA than did patients In other groups. Antibody titres of 0.40 ELISA units (EU) or more, or 0.35 EU or more detected 97% and 100% of staphylococcal LO-SVGIs, respectively, 0% and 2% titre/unit false-positive results. Our findings suggest that such an ELISA represents a sensitive, specific, and non-invasive diagnostic test for staphylococcal LO-SVGIs.

引用

页码：2166 / 2168

页数：3

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Henke PK, 1998, AM SURGEON, V64, P39

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