Continuous long-term somatic embryogenesis in alfalfa

被引：13

作者：

Tian, LN

Brown, DCW

Watson, E

机构：

[1] Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, London, ON N5V 4T3, Canada

[2] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2002年 / 38卷 / 03期

关键词：

embryogenic callus; Medicago sativa; plant regeneration; tissue culture;

D O I：

10.1079/IVP2001286

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Somatic embryogenic callus was induced on two induction media, B5h and SH4K. Embryos formed on the callus induced on B5h medium when the callus was still on the induction medium. On the other hand. embryos could not form on the callus induced on SH4K medium unless the callus was transferred to a growth regulator-free medium. Callus induced and maintained on B5h medium lost embryogenic capability quickly during the subculture. Callus induced and maintained on SH4K medium, however, consistently remained highly embryogenic. The callus mass showed steady increase during its maintenance on SH4K medium. The embryos induced on SH4K medium showed vigorous germination. Normal and fully fertile plants were recovered from the embryos developed from the callus maintained on SH4K medium.

引用

页码：279 / 284

页数：6

共 23 条

[1] PRODUCTION AND FIELD PERFORMANCE OF TRANSGENIC ALFALFA (MEDICAGO-SATIVA L) EXPRESSING ALPHA-AMYLASE AND MANGANESE-DEPENDENT LIGNIN PEROXIDASE [J].