Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis

被引:91
作者
Falbel, TG [1 ]
Meehl, JB [1 ]
Staehelin, LA [1 ]
机构
[1] UNIV COLORADO, DEPT MOL CELLULAR & DEV BIOL, BOULDER, CO 80309 USA
关键词
D O I
10.1104/pp.112.2.821
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Analyses of a series of allelic chlorina mutants of wheat (Triticum aestivum L.), which have partial blocks in chlorophyll (Chl) synthesis and, therefore, a limited Chl supply, reinforce the principle that Chl is required for the stable accumulation of Chl-binding proteins and that only reaction centers accumulate when the supply of Chl is severely limited. Depending on the rate of Chl accumulation (determined by the severity of the mutation) and on the rate of turnover of Chl and its precursors (determined by the environment in which the plant is grown), the mutants each reach an equilibrium of Chl synthesis and degradation. Together these mutants generate a spectrum of phenotypes. Under the harshest conditions (high illumination), plants with moderate blocks in Chl synthesis have membranes with very little Chl and Chl-proteins and membrane stacks resembling the thylakoids of the lethal xantha mutants of barley grown at low to medium light intensities (which have more severe blocks). In contrast, when grown under low-light conditions the same plants with moderate blocks have thylakoids resembling those of the wild type. The wide range of phenotypes of Chl b-deficient mutants has historically produced more confusion than enlightenment, but incomparable growth conditions can now explain the discrepancies reported in the literature.
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页码:821 / 832
页数:12
相关论文
共 63 条
[1]   REORGANIZATION OF THYLAKOID COMPONENTS DURING CHLOROPLAST DEVELOPMENT IN HIGHER-PLANTS AFTER TRANSFER TO DARKNESS - CHANGES IN PHOTOSYSTEM-I UNIT COMPONENTS, AND IN CYTOCHROMES [J].
AKOYUNOGLOU, A ;
AKOYUNOGLOU, G .
PLANT PHYSIOLOGY, 1985, 79 (02) :425-431
[2]  
Akoyunoglou G, 1978, CHLOROPLAST DEV, P843
[3]  
ALDRICH HC, 1986, ULTRASTRUCTURE TECHN, P101
[4]   BIOGENESIS OF THYLAKOID MEMBRANES IS CONTROLLED BY LIGHT-INTENSITY IN THE CONDITIONAL CHLOROPHYLL-B-DEFICIENT CD3 MUTANT OF WHEAT [J].
ALLEN, KD ;
DUYSEN, ME ;
STAEHELIN, LA .
JOURNAL OF CELL BIOLOGY, 1988, 107 (03) :907-919
[5]   RESOLUTION OF 16 TO 20 CHLOROPHYLL PROTEIN COMPLEXES USING A LOW IONIC-STRENGTH NATIVE GREEN GEL SYSTEM [J].
ALLEN, KD ;
STAEHELIN, LA .
ANALYTICAL BIOCHEMISTRY, 1991, 194 (01) :214-222
[6]   PHOTOREGULATION OF THE COMPOSITION, FUNCTION, AND STRUCTURE OF THYLAKOID MEMBRANES [J].
ANDERSON, JM .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1986, 37 :93-136
[7]  
Anderson JM., 1987, PHOTOINHIBITION, P1
[8]   A SUPRAMOLECULAR LIGHT-HARVESTING COMPLEX FROM CHLOROPLAST PHOTOSYSTEM-II MEMBRANES [J].
BASSI, R ;
DAINESE, P .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 204 (01) :317-326
[9]   CHLOROPHYLL-PROTEIN COMPLEXES OF BARLEY PHOTOSYSTEM-I [J].
BASSI, R ;
SIMPSON, D .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 163 (02) :221-230
[10]  
BELLEMARE G, 1982, J BIOL CHEM, V257, P7762