The Neurospora crassa chs3 gene encodes an essential class I chitin synthase

被引:21
作者
Beth-Din, A [1 ]
Yarden, O [1 ]
机构
[1] Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Dept Plant Pathol & Microbiol, IL-76100 Rehovot, Israel
关键词
cell wall; growth; development;
D O I
10.2307/3761451
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In Neurospora crassa, at least five different DNA fragments related to chitin synthases have been identified. In this study we have cloned, sequenced, mapped and characterized the chitin synthase 3 structural gene (designated chs3) of N. crassa. Based on the amino acid sequence deduced from the cloned chs3 genomic DNA fragments we have designated this gene as a class I chitin synthase. The chs3 gene is transcribed predominantly at the early stages of mycelial growth and is expressed during all stages of asexual growth. The fact that strains with nuclei harboring a disrupted chs3 gene could only be maintained as heterokaryons indicates that this gene is indispensable in N. crassa.
引用
收藏
页码:65 / 73
页数:9
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