Immunohistochemical localization of the LH/HCG receptor in human ovary: HCG enhances cell surface expression of LH/HCG receptor on luteinizing granulosa cells in vitro

被引:38
作者
Takao, Y
Honda, T
Ueda, M
Hattori, N
Yamada, S
Maeda, M
Fujiwara, H
Mori, T
Wimalasena, J
机构
[1] KYOTO UNIV, DEPT GYNECOL & OBSTET, FAC MED, SAKYO KU, KYOTO 60601, JAPAN
[2] KYOTO UNIV, INST VIRUS RES, SAKYO KU, KYOTO 60601, JAPAN
[3] KYOTO UNIV, CHEST DIS RES INST, SAKYO KU, KYOTO 60601, JAPAN
[4] UNIV TENNESSEE, DEPT OBSTET & GYNECOL, KNOXVILLE, TN USA
关键词
luteinizing hormone; ovarian cells; receptor;
D O I
10.1093/molehr/3.7.569
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We examined the immunohistochemical localization of luteinizing hormone (LH)/human chorionic gonadotrophin (HCG) receptor (LH-R) in the human ovary using the anti-human LH-R monoclonal antibody, 3B5. In the antral follicles, LH-R was detected on theca interna cells. In pre-ovulatory follicles, granulosa cells also expressed LH-R. During corpus luteum formation, granulosa cells seemed to increase the expression of LH-R, and in corpus luteum of mid-luteal phase, large luteal cells expressed LH-R more intensely than small luteal cells. In the regressing corpus luteum, LH-R was almost undetectable on both luteal cells, whereas in the corpus luteum of early pregnancy, LH-R continued to be expressed on large luteal cells. The granulosa cells obtained from the patients undergoing in-vitro fertilization therapy were cultured for 3 days in serum-free medium, without or with HCG (10 IU/ml) and tumour necrosis factor (TNF)alpha (10 ng/ml). Flow cytometry showed that the expression of LH-R on the cell surface of luteinizing granulosa cells was enhanced by HCG, but was unaffected by TNF alpha. These results suggest that the main target cells for LH/HCG change from theca interna cells/small luteal cells to granulosa cells/large luteal cells during ovulation, corpus luteum formation, and differentiation into the corpus luteum of pregnancy, probably under the influence of LH/HCG.
引用
收藏
页码:569 / 578
页数:10
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