Rheological characterization of schizophyllan aqueous solutions after denaturation-renaturation treatment

被引:30
作者
Fang, YP [1 ]
Takahashi, R [1 ]
Nishinari, K [1 ]
机构
[1] Osaka City Univ, Grad Sch Human Life Sci, Dept Food & Human Hlth Sci, Sumiyoshi, Osaka 5588585, Japan
关键词
schizophyllan; denatured-renatured; weak gel; aggregate; critical overlap; thixotropy;
D O I
10.1002/bip.20081
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Schizophyllan (SPG) with a molecular weight of 2.6 x 10(6), designated SPG-1, is denatured and then renatured at a concentration of 1.8 wt % by alkalization-neutralization. The prepared denatured-renatured samples (DRSPG-1) are diluted to various concentrations and equilibrated for 10 days before rheological and intrinsic viscosity measurements. When concentration (C-p) is above 0.75 wt %, DRSPG-1 aqueous systems have weak gel-type rheological properties. However, for 0.28 wt % less than or equal to C-p less than or equal to 0.65 wt % and C-p less than or equal to 0.19 wt %, DRSPG-1 aqueous systems behave as power law fluids and Newtonian fluids, respectively, which are attributed to the moderate isotropy degree of DRSPG-1 chains. Furthermore, a critical overlap parameter of c*[eta] = 1.2 is determined for DRSPG-1 in aqueous solutions, which is close to that of I for intact SPG in water while far smaller than that of 4.3 for SPG in DMSO. This is considered to be due to the strong interactions of DRSPG-1 chains in water, further confirmed by the intrinsic viscosity measurements in which the DRSPG-1 aqueous solution shows an abnormally large value of Huggins constant. Regarding the structure of DRSPG-1 weak gels, multiruns of dynamic strain sweep measurements suggest that the dominant structures are aggregates formed by hydrogen-bonding associations of DRSPG-1 chains rather than the permanent three-dimensional network. In addition, the step-shear rate tests are performed to study the thixotropic properties of DRSPG-1 aqueous systems. (D 2004 Wiley Periodicals, Inc.
引用
收藏
页码:302 / 315
页数:14
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