Method for rapid purification of class IIa bacteriocins and comparison of their activities

被引:96
作者
Guyonnet, D
Fremaux, C
Cenatiempo, Y
Berjeaud, JM
机构
[1] IBMIG, Mol Biol Lab, CNRS ESA 6031, F-86022 Poitiers, France
[2] RHODLA FOOD, F-86220 Dange St Romain, France
关键词
D O I
10.1128/AEM.66.4.1744-1748.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A three-step method nas developed for the purification of mesentericin Y105 (60% yield) from the culture supernatant of Leuconostoc mesenteroides Y105. The same procedure was successfully applied to the purification of fire other anti-Listeria bacteriocins identified by mass spectrometry. Specific activities of the purified bacteriocins were compared.
引用
收藏
页码:1744 / 1748
页数:5
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