Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements

被引：116

作者：

Lundby, Alicia ^{[1
,2
]}

Mutoh, Hiroki ^{[1
]}

Dimitrov, Dimitar ^{[1
]}

Akemann, Walther ^{[1
]}

Knopfel, Thomas ^{[1
]}

机构：

[1] RIKEN, Brain Sci Inst, Lab Neuronal Circuit Dynam, Wako, Saitama, Japan

[2] Univ Copenhagen, Danish Natl Res Fdn Ctr Cardiac Arrhythmia, Copenhagen, Denmark

来源：

PLOS ONE | 2008年 / 3卷 / 06期

关键词：

D O I：

10.1371/journal.pone.0002514

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

引用

页数：5

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