High-throughput verification of transcriptional starting sites by Deep-RACE

被引：28

作者：

Olivarius, Signe ^{[1
]}

Plessy, Charles ^{[1
]}

Carninci, Piero ^{[1
]}

机构：

[1] RIKEN, Yokohama Inst, Om Sci Ctr, Funct Genom Technol Team,Tsurumi Ku, Kanagawa 2300045, Japan

来源：

BIOTECHNIQUES | 2009年 / 46卷 / 02期

关键词：

GENOME;

D O I：

10.2144/000113066

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep-rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes an is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.

引用

页码：130 / 132

页数：3

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