Role of TGF-β1 expressed in bone marrow-derived mesenchymal stem cells in promoting bone formation in a rabbit femoral defect model

被引：27

作者：

Sun, Bing-Yin ^{[1
]}

Zhao, Bao-Xiang ^{[2
]}

Zhu, Jie-Ying ^{[3
]}

Sun, Zheng-Ping ^{[4
]}

Shi, Yong-An ^{[5
]}

Huang, Feng ^{[6
]}

机构：

[1] Guangzhou Univ Chinese Med, Dept Orthoped, Shunde Hosp, Foshan 528200, Guangdong, Peoples R China

[2] Linyi Peoples Hosp, Dept Orthoped, Linyi 276000, Shandong, Peoples R China

[3] Guangzhou Med Univ, Dept Subst Dependence, Affiliated Brain Hosp, Guangzhou Huiai Hosp, Guangzhou 510370, Guangdong, Peoples R China

[4] Second Tradit Chinese Med Hosp Guangdong Provence, Phys Examinat Ctr, Guangzhou 510095, Guangdong, Peoples R China

[5] Wendeng Hosp Tradit Chinese Orthoped & Traumatol, Dept Microsurg 2, Wendeng 264400, Shandong, Peoples R China

[6] Guangzhou Univ Tradit Chinese Med, Dept Orthoped, Affiliated Hosp 1, 16 Jichang Rd, Guangzhou 510405, Guangdong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2018年 / 42卷 / 02期

关键词：

transforming growth factor-beta 1; bone marrow-derived mesenchymal stem cells; rabbit femoral defect; new bone formation; GROWTH-FACTOR-BETA; TGF-BETA; DISTRACTION OSTEOGENESIS; REGENERATIVE MEDICINE; STROMAL CELLS; DIFFERENTIATION; PROTEOLYSIS; PROTEIN; MATRIX; INJURY;

D O I：

10.3892/ijmm.2018.3692

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Bone defects represent a major clinical and socioeconomic problem without suitable treatment options. Previous studies have shown that transforming growth factor 1 (TGF-beta 1) is important in the development of various diseases. The present study aimed to investigate the therapeutic potential of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) expressing TGF-beta 1 in the treatment of rabbit femoral defects. First, rabbit BMSCs were identified and cultured. TGF-beta 1 was then stably overexpressed in the rabbit BMSCs by lentivirus transfection, which was expressed at a high level in the femoral defects treated with TGF-beta 1-overexpressing BMSCs, compared with PBS-treated controls. In addition, the TGF-beta 1-overexpressing BMSCs promoted new bone formation in the rabbit femoral defect model, and increased the expression of bone-related markers at week 2 and week 6. Therefore, the study demonstrated that BMSCs overexpressing TGF-beta 1 may provide a novel therapeutic option for femoral defects.

引用

页码：897 / 904

页数：8

共 35 条

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