Effects of Moritella viscosa antigens on pro-inflammatory gene expression in an Atlantic salmon (Salmo salar Linnaeus) cell line (SHK-1)

被引：16

作者：

Bjornsdottir, Bryndis ^{[1
,2
]}

Fast, Mark D. ^{[2
]}

Sperker, Sandra A. ^{[2
]}

Brown, Laura L. ^{[2
]}

Gudmundsdottir, Bjarnheidur K. ^{[1
]}

机构：

[1] Univ Iceland, Inst Expt Pathol, IS-112 Reykjavik, Iceland

[2] Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 2Z1, Canada

来源：

FISH & SHELLFISH IMMUNOLOGY | 2009年 / 26卷 / 06期

关键词：

Moritella viscosa; Extracellular products; MvP1; vibriolysin; Atlantic salmon; SHK-1; Gene expression; Immune response; Pro-inflammatory cytokine; Interleukin-1 beta (IL-1 beta); Interleukin-8 (IL-8); VIBRIO-VISCOSUS; RAINBOW-TROUT; WINTER ULCER; HEAD KIDNEY; FISH; EVOLUTION; CYTOKINES; L; INTERLEUKIN-1-BETA; VACCINATION;

D O I：

10.1016/j.fsi.2009.03.015

中图分类号：

S9 [水产、渔业];

学科分类号：

090805 [渔业资源学];

摘要：

Moritella viscosa is the causative agent of winter ulcer disease in salmonids reared in North-Atlantic countries. In this study the effects of selected M. viscosa antigens on cytotoxicity and pro-inflammatory gene expression in an Atlantic salmon (Salmo salar Linnaeus) macrophage-like cell line (SHK-1) were examined. SHK-1 cells were stimulated with live and heat-killed bacterial cells, extracellular products (ECP) and an extracellular vibriolysin, termed MvP1. Following incubation, cytotoxicity and expression levels of interleukin-1 beta (IL-1 beta) and interleukin-8 (IL-8) were examined at different time points. Both live M. viscosa cells and ECP were cytotoxic, but neither heat-killed cells, nor the MvP1 peptidase caused cell death. Expression levels of both IL-1 beta and IL-8 increased significantly after stimulation with live cells, but heat-killed cells only caused increased IL-8 expression. ECP did not affect IL-1 beta expression, but did stimulate IL-8 expression. The isolated MvP1 peptidase stimulated both IL-1 beta and IL-8 expression at the highest concentration tested. This study reveals a difference in the induction of pro-inflammatory gene expression in salmon SHK-1 cells between live and heat-killed M. viscosa cells, and also that an unknown secreted factor is the main stimulant of IL-beta and IL-8 expression. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：858 / 863

页数：6

共 27 条

[1]

Characterization of Vibrio viscosus and Vibrio wodanis isolated at different geographical locations:: a proposal for reclassification of Vibrio viscosus as Moritella viscosa comb. nov. [J].

Benediktsdóttir, E ;

Verdonck, L ;

Spröer, C ;

Helgason, S ;

Swings, J .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :479-488

[2]

Vibrio spp. isolated from salmonids with shallow skin lesions and reared at low temperature [J].

Benediktsdottir, E ;

Helgason, S ;

Sigurjonsdottir, H .

JOURNAL OF FISH DISEASES, 1998, 21 (01) :19-28

[3]

Evolution of interleukin-1β [J].

Bird, S ;

Zou, J ;

Wang, TH ;

Munday, B ;

Cunningham, C ;

Secombes, CJ .

CYTOKINE & GROWTH FACTOR REVIEWS, 2002, 13 (06) :483-502

[4]

Characterisation of an extracellular vibriolysin of the fish pathogen Moritella viscosa [J].

Bjornsdottir, Bryndis ;

Fridjonsson, Olafur H. ;

Magnusdottir, Steinunn ;

Andresdottir, Valgerdur ;

Hreggvidsson, Gudmundur O. ;

Gudmundsdottir, Bjarnheidur K. .

VETERINARY MICROBIOLOGY, 2009, 136 (3-4) :326-334

[5]

Vibrio viscosus in farmed Atlantic salmon Salmo salar in Scotland:: field and experimental observations [J].

Bruno, DW ;

Griffiths, J ;

Petrie, J ;

Hastings, TS .

DISEASES OF AQUATIC ORGANISMS, 1998, 34 (03) :161-166

[6]

Characterisation of a long-term cell line (SHK-1) developed from the head kidney of Atlantic salmon (Salmo salar L) [J].

Dannevig, BH ;

Brudeseth, BE ;

Gjoen, T ;

Rode, M ;

Wergeland, HI ;

Evensen, O ;

Press, CM .

FISH & SHELLFISH IMMUNOLOGY, 1997, 7 (04) :213-226

[7]

Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation [J].

Fast, M. D. ;

Johnson, S. C. ;

Eddy, T. D. ;

Pinto, D. ;

Ross, N. W. .

PARASITE IMMUNOLOGY, 2007, 29 (04) :179-189

[8]

Differential expression of the pro-inflammatory cytokines IL-1β-1, TNFα-1 and IL-8 in vaccinated pink (Oncorhynchus gorbuscha) and chum (Oncorhynchus keta) salmon juveniles [J].

Fast, M. D. ;

Johnson, S. C. ;

Jones, S. R. M. .

FISH & SHELLFISH IMMUNOLOGY, 2007, 22 (04) :403-407

[9]

Cortisol response and immune-related effects of Atlantic salmon (Salmo salar Linnaeus) subjected to short- and long-term stress [J].

Fast, Mark D. ;

Hosoya, Sho ;

Johnson, Stewart C. ;

Afonso, Luis O. B. .

FISH & SHELLFISH IMMUNOLOGY, 2008, 24 (02) :194-204

[10]

Prostaglandin E2 modulation of gene expression in an Atlantic salmon (Salmo salar) macrophage-like cell line (SHK-1) [J].

Fast, MD ;

Ross, NW ;

Johnson, SC .

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2005, 29 (11) :951-963

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