Association of a lipoxygenase locus, Lpx-B1, with variation in lipoxygenase activity in durum wheat seeds

被引:70
作者
Hessler, TG
Thomson, MJ
Benscher, D
Nachit, MM
Sorrells, ME
机构
[1] Cornell Univ, Dept Plant Breeding, Ithaca, NY 14853 USA
[2] ICARDA, CIMMYT, Durum Improvement Program, Aleppo, Syria
关键词
D O I
10.2135/cropsci2002.1695
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Lipoxygenases constitute a family of enzymes that catalyze the breakdown of lipids, resulting in products that may have undesirable effects. These enzymes can affect pasta color and cause off-flavors. The purpose of this study was to map the loci associated with lipoxygenase activity in seeds of a durum wheat (Triticum turgidum ssp. durum) population to determine if lipoxygenase activity is associated with flour color. Seed of the parents, Jennah Khetifa and Cham 1, and the recombinant inbred population consisting of 113 progeny lines were used to assay lipoxygenase activity and flour color. A saturated molecular-marker linkage map for this population was previously constructed. Allele specific primers targeting the wheat homolog to barley LoxA were designed, and a fragment length polymorphism resulting from a miniature inverted-repeat transposable element (MITE) insertion in an intron of the durum wheat LoxA homolog allowed mapping of the locus to chromosome 4BS. To measure lipoxygenase activity, spectrophotometric changes were recorded. Chain I had a change in absorbance of 0.284 units of absorbance over I min compared with 0.752 units for Jennah Khetifa. Quantitative trait locus analysis of these data indicated that most of the lipoxygenase activity was associated with the wheat lipoxygenase gene (Lpx) on chromosome 4BS. A quantitative trait locus, for vitreous appearance of seeds also was associated with this locus. Flour color was not correlated with lipoxygenase activity in this population. With this knowledge, marker-assisted selection can be used to select genotypes with lower lipoxygenase activity in durum wheat populations.
引用
收藏
页码:1695 / 1700
页数:6
相关论文
共 38 条
[1]   Durum wheat lipoxygenase activity and other quality parameters that affect pasta color [J].
Borrelli, GM ;
Troccoli, A ;
Di Fonzo, N ;
Fares, C .
CEREAL CHEMISTRY, 1999, 76 (03) :335-340
[2]   STOWAWAY - A NEW FAMILY OF INVERTED REPEAT ELEMENTS ASSOCIATED WITH THE GENES OF BOTH MONOCOTYLEDONOUS AND DICOTYLEDONOUS PLANTS [J].
BUREAU, TE ;
WESSLER, SR .
PLANT CELL, 1994, 6 (06) :907-916
[3]   The MITE family Heartbreaker (Hbr):: Molecular markers in maize [J].
Casa, AM ;
Brouwer, C ;
Nagel, A ;
Wang, LJ ;
Zhang, Q ;
Kresovich, S ;
Wessler, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (18) :10083-10089
[4]  
DROST B W, 1990, Journal of the American Society of Brewing Chemists, V48, P124
[5]  
Furuta S., 1996, J AGR FOOD CHEM, V44, P236
[6]   IDENTIFICATION OF A NOVEL BETA-TURN-RICH REPEAT MOTIF IN THE D-HORDEINS OF BARLEY [J].
HALFORD, NG ;
TATHAM, AS ;
SUI, E ;
DARODA, L ;
DREYER, T ;
SHEWRY, PR .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1122 (02) :118-122
[7]   CHROMOSOMAL LOCATION AND EVOLUTION OF ISOENZYME STRUCTURAL GENES IN HEXAPLOID WHEAT [J].
HART, GE ;
LANGSTON, PJ .
HEREDITY, 1977, 39 (OCT) :263-+
[8]   LIPOXYGENASES [J].
HILDEBRAND, DF .
PHYSIOLOGIA PLANTARUM, 1989, 76 (02) :249-253
[9]   Differential expression of lipoxygenase isoenzymes in embryos of germinating barley [J].
Holtman, WL ;
vanDuijn, G ;
Sedee, NJA ;
Douma, AC .
PLANT PHYSIOLOGY, 1996, 111 (02) :569-576
[10]  
Irvine G., 1971, WHEAT CHEM TECHNOLOG, P777