Antimicrobial activity of marine bacteria associated with sponges from the waters off the coast of South East India

被引:116
作者
Anand, T. Prem [1 ]
Bhat, Abdul Wajid
Shouche, Yogesh S.
Roy, Upal
Siddharth, Jay
Sarma, Siddhartha P.
机构
[1] Indian Inst Sci, Mol Biophys Unit, Bangalore 60012, Karnataka, India
[2] Univ Pune, Natl Ctr Cell Sci, Pune 11007, Maharashtra, India
关键词
antimicrobials; marine bacteria; marine sponge; phylogeny; India;
D O I
10.1016/j.micres.2005.09.002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Seventy-five marine bacterial. strains associated with four species of sponges (Echinodictyum sp., Spongia sp., Sigmadocia fibulatus and Mycale mannarensis) were isolated from the Tuticorin coast, Gulf of Mannar region. The agar-overlay method was used to screen for antibiotic production by these strains against four bacteria, viz., Bacillus subtilis, Escherichia coli, Vibrio parahaemolyticus, and Vibrio harveyi and one fungal pathogen, viz., Candida albicans. Twenty-one per cent of the bacterial. strains were found to be antibiotic producers and their activities ranged from broad spectral to species specific. A strain coded SC3 was found to be highly potent and was mass cultured. The ethyl acetate extract of the culture broth was further fractionated by reverse phase HPLC and the active fraction identified. In addition, SC3 was subjected to morphological and physiological characterization. The results of the tests showed SC3 to be a Gram-positive rod, sporutating, motite, catatase and oxiclase positive. Phytogenetic analysis based on comparative analysis of sequenced 16s rRNA of the active strains indicated a preponderance of bacteria belonging to Vibrio and Bacillus genera with 95-99% sequence similarities. To our knowledge this is the first report on phytogenetic identification of antibiotic producing bacteria associated with sponges from Indian waters. (c) 2005 Etsevier GmbH. All rights reserved.
引用
收藏
页码:252 / 262
页数:11
相关论文
共 45 条
[1]  
Anand TP, 1997, INDIAN J MAR SCI, V26, P206
[2]  
Anand TP, 2002, INDIAN J MAR SCI, V31, P239
[3]  
Bernan VS, 1997, ADV APPL MICROBIOL, V43, P57, DOI 10.1016/S0065-2164(08)70223-5
[4]   Metabolites from the sponge-associated bacterium Pseudomonas species [J].
Bultel-Poncé, V ;
Berge, JP ;
Debitus, C ;
Nicolas, JL ;
Guyot, M .
MARINE BIOTECHNOLOGY, 1999, 1 (04) :384-390
[5]   Microbial antagonism: a neglected avenue of natural products research [J].
Burgess, JG ;
Jordan, EM ;
Bregu, M ;
Mearns-Spragg, A ;
Boyd, KG .
JOURNAL OF BIOTECHNOLOGY, 1999, 70 (1-3) :27-32
[6]  
Burja Adam M., 1999, Memoirs of the Queensland Museum, V44, P63
[7]   Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method [J].
Callewaert, R ;
Holo, H ;
Devreese, B ;
Van Beeumen, J ;
Nes, I ;
De Vuyst, L .
MICROBIOLOGY-SGM, 1999, 145 :2559-2568
[8]  
Chandramohan D, 1997, J MAR BIOTECHNOL, V5, P73
[9]   Marine bacteria associated with sponge as source of cyclic peptides [J].
De Rosa, S ;
Mitova, M ;
Tommonaro, G .
BIOMOLECULAR ENGINEERING, 2003, 20 (4-6) :311-316
[10]  
DOBLER IW, 2002, ADV BIOCHEM ENG BIOT, V74, P207